Experimental study on subchronic toxicity of Guizhou produced Rosa sterilis var. leioclada in SD rats

Abstract

Abstract:

Objective To investigate the toxic effects of Rosa sterilis var. leioclada on SD rats，identify target organs，provide the estimation of the adverse effect level，and provide a research basis for the development of the characteristic plant resources as food ingredients. Methods Based on the results of the median lethal dose（LD₅₀） test，according to the "National standard of food safety 90-day oral toxicity test"，using Rosa sterilis var. leiocladaas the test material，SD rats as the experimental animals，set up four dosage groups of 0.0，10.0（1/16 LD₅₀），20.0（1/8 LD₅₀） and 40.0（1/4LD₅₀）g/（kg·bw），and each rat was orally gavaged once a day according to the gavage volume of 10 g/kg body weight，and continuously fed for 90 d. The rats were examined for body weight，food intake，histopathological changes，and blood physiological and biochemical indexes. Results Compared with the control group，the body weight，food intake and food utilization rate of rats in the experimental group did not change，and the differences were not statistically significant（all P>0.05）.The urine PH，urine specific gravity，urine glucose of rats in some experimental groups changed，and the differences were statistically significant（all P<0.05）.The blood alkaline phosphatase and glutamic oxaloacetic aminase content of rats in some experimental groups changed，and the differences were statistically significant（both P<0.05）.Changes in blood lymphocyte percentage，neutrophil ratio，platelet and leukocyte count in some experimental groups of rats showed statistically significant differences（all P<0.05）.Changes in uterine weight，uterine body mass ratio，testicle weight in some experimental groups of rats showed statistically significant differences（all P<0.05）.No adverse changes related to the test substance were observed in the histopathological examination of rats in the high-dose group. The actual intake of Rosa sterilis var. leioclada in female and male rats did not observe harmful effects at doses not less than 40 000 mg/kg. Conclusion Under the experimental dosage and intervention period，no pathological tissue changes were observed in rats，and there was no negative effect on the blood biochemical level of rats，suggesting that the Rosa sterilis var. leioclada is non-toxic，has no sub-chronic toxicity，and has no potential risk of safety in consumption.

Key words: Rosa sterilis var. leioclada, 90-day feeding experiment, Safety evaluation

CLC Number:

R114

WU Maorui, LIU Yongting, ZHENG Chong, LIN Ye, YIN Mingyue, LIU Liya. Experimental study on subchronic toxicity of Guizhou produced Rosa sterilis var. leioclada in SD rats. [J]OCCUPATION AND HEALTH, 2026, 42(2): 161-167.

Figures/Tables 12

References 25

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性别	剂量组	初始体质量（g）	终体质量（g）	体质量增长量（g）	总进食量（g）	总食物利用率（%）
雌	高剂量组	92.60±7.21	308.30±29.92	215.70±31.39	1 692.20±242.80	12.72±0.68
	中剂量组	92.20±7.45	327.00±27.12	234.80±24.80	1 885.00±146.32	12.45±0.71
	低剂量组	91.50±6.87	319.00±40.34	227.50±39.06	1 769.90±207.75	12.79±0.89
	对照组	90.60±7.11	315.10±29.52	224.50±29.61	1 751.90±132.35	12.78±0.99
雄	高剂量组	105.70±6.55	542.08±60.69	469.70±72.38	2 393.00±366.66	19.79±3.12
	中剂量组	106.10±5.95	576.10±38.39	470.00±37.71	2 383.50±148.02	19.72±1.09
	低剂量组	106.20±6.88	584.90±33.70	478.70±38.47	2 453.30±130.62	19.51±1.11
	对照组	105.80±6.43	597.20±51.60	491.40±53.06	2 554.90±208.80	19.21±0.98

性别	剂量组	初始体质量（g）	终体质量（g）	体质量增长量（g）	总进食量（g）	总食物利用率（%）
雌	高剂量组	92.60±7.21	308.30±29.92	215.70±31.39	1 692.20±242.80	12.72±0.68
	中剂量组	92.20±7.45	327.00±27.12	234.80±24.80	1 885.00±146.32	12.45±0.71
	低剂量组	91.50±6.87	319.00±40.34	227.50±39.06	1 769.90±207.75	12.79±0.89
	对照组	90.60±7.11	315.10±29.52	224.50±29.61	1 751.90±132.35	12.78±0.99
雄	高剂量组	105.70±6.55	542.08±60.69	469.70±72.38	2 393.00±366.66	19.79±3.12
	中剂量组	106.10±5.95	576.10±38.39	470.00±37.71	2 383.50±148.02	19.72±1.09
	低剂量组	106.20±6.88	584.90±33.70	478.70±38.47	2 453.30±130.62	19.51±1.11
	对照组	105.80±6.43	597.20±51.60	491.40±53.06	2 554.90±208.80	19.21±0.98

剂量组	红细胞计数（×10¹²个/L）	白细胞计数（×10⁹个/L）	淋巴细胞（%）	中性粒细胞（%）	血红蛋白浓度（g/L）	红细胞压积（%）	血小板（×10⁹个/L）	凝血酶原时间（s）	活化部分凝血酶原时间（s）
高剂量组	7.99±0.95	2.75±1.17	79.36±5.82^a	16.26±5.94^a	150.00±20.39	43.77±4.75	762.30±121.26^a	11.54±0.45	11.40±0.43
中剂量组	8.03±0.33	3.22±0.94	80.63±5.45	14.98±5.56	153.80±5.65	45.22±1.83	844.60±83.59	11.25±0.51	12.85±3.44
低剂量组	7.89±0.48	3.07±0.98	80.95±5.12	12.99±4.39	146.90±7.42	43.39±2.27	836.50±87.50	11.55±0.44	12.14±1.01
对照组	8.09±0.29	3.60±1.53	85.43±4.16	10.52±3.13	150.30±7.24	44.18±1.86	881.30±101.93	11.35±0.40	12.52±3.01
F值	0.206	0.892	0.574	2.631	2.618	0.722	2.505	1.073	0.705
P值	0.891	0.455	0.636	0.065	0.066	0.546	0.075	0.373	0.555

剂量组	红细胞计数（×10¹²个/L）	白细胞计数（×10⁹个/L）	淋巴细胞（%）	中性粒细胞（%）	血红蛋白浓度（g/L）	红细胞压积（%）	血小板（×10⁹个/L）	凝血酶原时间（s）	活化部分凝血酶原时间（s）
高剂量组	7.99±0.95	2.75±1.17	79.36±5.82^a	16.26±5.94^a	150.00±20.39	43.77±4.75	762.30±121.26^a	11.54±0.45	11.40±0.43
中剂量组	8.03±0.33	3.22±0.94	80.63±5.45	14.98±5.56	153.80±5.65	45.22±1.83	844.60±83.59	11.25±0.51	12.85±3.44
低剂量组	7.89±0.48	3.07±0.98	80.95±5.12	12.99±4.39	146.90±7.42	43.39±2.27	836.50±87.50	11.55±0.44	12.14±1.01
对照组	8.09±0.29	3.60±1.53	85.43±4.16	10.52±3.13	150.30±7.24	44.18±1.86	881.30±101.93	11.35±0.40	12.52±3.01
F值	0.206	0.892	0.574	2.631	2.618	0.722	2.505	1.073	0.705
P值	0.891	0.455	0.636	0.065	0.066	0.546	0.075	0.373	0.555

剂量组	红细胞计数（×10¹²个/L）	白细胞计数（×10⁹个/L）	淋巴细胞（%）	中性粒细胞（%）	血红蛋白浓度（g/L）	红细胞压积（%）	血小板（×10⁹个/L）	凝血酶原时间（s）	活化部分凝血酶原时间（s）
高剂量组	8.72±0.51	4.90±1.18^a	79.69±4.09	15.66±2.61	153.60±7.40	43.58±2.21	956.50±129.63	11.80±0.99	11.33±0.31
中剂量组	8.98±0.45	6.09±2.20	79.46±8.06	16.42±7.61	156.60±9.02	43.73±1.92	1 005.10±126.65	11.91±0.33	11.24±0.05
低剂量组	9.07±0.80	6.64±1.87	81.25±6.21	13.96±5.27	157.30±9.43	44.72±3.07	936.90±81.37	12.23±0.62	12.36±3.53
对照组	8.65±0.40	7.92±2.26	83.86±3.80	11.65±4.11	153.50±6.85	43.26±1.80	990.50±222.69	14.16±4.13	12.02±2.39
F值	1.310	4.230	1.219	1.638	0.579	0.747	0.436	2.636	0.644
P值	0.286	0.012	0.317	0.198	0.633	0.531	0.729	0.064	0.592