Objective To investigate the current status of technical stress,emotional intelligence and job burnout among internal medicine nurses and analyze the correlation between the three,explore the mediating role of emotional intelligence between technical stress and job burnout,and provide reference evidence for reducing job burnout of internal medicine nurses and improving clinical nursing work efficiency. Methods A random sampling method was used to conduct a cross-sectional survey on 176 internal medicine nurses from 9 grade-A tertiary hospitals in Wuhan from May to June 2025 using technical stress scale,emotional intelligence scale and job burnout scale. Pearson correlation analysis was used to analyze the correlation between technical stress,emotional intelligence and job burnout of internal medicine nurses. The mediation effect was tested to determine the strength of the mediation effect and the Bootstrap method was used to verify it. Results The technical stress score of internal medicine nurses was (30.42±5.84)points,the emotional intelligence score was (72.86±14.28)points,and the job burnout score was (58.92±13.64)points. The technical stress of internal medicine nurses was related to their age,educational level,total clinical nursing work hours,nursing professional title,number of children,average monthly night shifts,and marital status,with statistically significant differences(all P<0.05). The emotional intelligence and job burnout of internal medicine nurses were related to their age,total clinical nursing work hours,nursing professional title,number of children,and average monthly night shifts,with statistically significant differences(all P<0.05). Technical stress was positively correlated with job burnout(r=0.574,P<0.01),emotional intelligence was negatively correlated with job burnout(r=-0.536,P<0.01),and technical stress was negatively correlated with emotional intelligence(r=-0.489,P<0.01). The emotional intelligence had a partial mediating effect between technical stress and job burnout of internal medicine nurses,with a mediation effect value of 0.318,accounting for 47.32% of the total effect. Conclusion Internal medicine nurses have high technical stress,which can positively predict their job burnout. Emotional intelligence plays a partial mediating role between the two. Improving emotional intelligence can help alleviate the impact of technical stress on job burnout.