Objective To investigate the current status of occupational burnout,work-non-work boundary penetration and work-family conflict among dermatology nurses and study the interaction among the three,explore the mediating role of work-family conflict,and provide a reference for formulating intervention measures to reduce occupational burnout of dermatology nurses and improve clinical nursing work efficiency. Methods From April to May 2025,a random sampling method was used to survey 174 dermatology nurses from 5 grade-A tertiary general hospitals in Chengdu using the work-non-work boundary penetration scale,work-family conflict scale and occupational burnout scale,and the data were analyzed. Results The work-non-work boundary penetration score of dermatology nurses was (48.83±7.64)points,work-family conflict score was (58.74±9.25)points,and occupational burnout score was (60.32±12.54)points. The scores of work-non-work boundary penetration,work-family conflict,and occupational burnout of dermatology nurses with different clinical nursing professional titles,ages,average monthly night shift frequency,average monthly total salary,and marital status were compared,and the differences were statistically significant(all P<0.05). Work-non-work boundary penetration was positively correlated with burnout(r=0.591,P<0.01),work-family conflict was positively correlated with burnout(r=0.629,P<0.01),and work-non-work boundary permeability was positively correlated with work-family conflict(r=0.635,P<0.01). Work-family conflict partially mediated the relationship between work-non-work boundary permeability and burnout among dermatology nurses,with a mediating effect value of 0.208,accounting for 35.74% of the total effect. Conclusion Work-non-work boundary permeability,work-family conflict,and burnout all at relatively high levels among dermatology nurses,and work-family conflict partially mediates the relationship between work-non-work boundary permeability and burnout. Reducing work-family conflict and work-non-work boundary permeability among dermatology nurses can help improve their burnout and enhance clinical nursing efficiency.