Analysis of pathogenic Vibrio contamination in aquatic products in Cangzhou City of Hebei Province in 2021

Abstract

Abstract: Objective To investigate the pollution status of pathogenic Vibrio in aquatic products in Cangzhou City，provide data support for the prevention and control of foodborne diseases and monitoring of food poisoning. Methods The samples of aquatic products in Cangzhou City in 2021 were collected by random sampling. According to the methods of National work manual for risk monitoring of food contamination and harmful factors，four kinds of pathogenic Vibrio were detected，the virulence genes of Vibrio cholerae and Vibrio parahaemolyticus were determined，and the Vibrio cholerae was serotyped. Results The pathogenic Vibrio was detected in 74 of 220 aquatic products，and the detection rate was 33.64%. The detection rate of Vibrio parahaemolyticus，Vibrio alginolyticus，Vibrio cholerae and Vibrio vulnificus was 22.73%，7.27%，2.73% and 0.91%，respectively. All 50 strains of Vibrio parahaemolyticus isolated carried the tlh genotype，but did not carry the tdh and trh genes. The six strains of Vibrio cholerae were non-O1/O139 group，and the ctx virulence gene was negative. Conclusions In 2021，there is pathogenic Vibrio contamination in some aquatic products in Cangzhou City，mainly Vibrio parahaemolyticus and Vibrio alginolyticus. The market supervision department should strengthen the supervision of various aquatic products to reduce the risk of foodborne diseases caused by pathogenic Vibrio.

Key words: Aquatic products, Pathogenic vibrio, Pollution status, Virulence genes

CLC Number:

R155.5

WU Bing-lun, ZHANG Rui, BI Hong-jie, LU Wei, HU Qian-qian, WANG Ju-zhong. Analysis of pathogenic Vibrio contamination in aquatic products in Cangzhou City of Hebei Province in 2021. [J]OCCUPATION AND HEALTH, 2023, 39(3): 326-328.

References

[1] 卢俊荣,王春东,毕红杰,等.河北省沧州市2012年全年市售食品食源性致病菌污染状况调查[J].山西医药杂志(下半月刊),2013,42(3):269-270.
[2] 吴莹,刘军,王银平,等.淄博市300份市售动物性水产品中主要致病性弧菌毒力基因检测[J].现代预防医学,2019,46(12):2167-2170.
[3] 国家食品药品监督管理总局.食品安全国家标准食品微生物学检验总则:GB 4789.1—2016[S].北京:中国质检出版社,2017:1-12.
[4] 李莹,闫琳,杨舒然,等.《国家食品污染和有害因素风险监测工作手册》[M].北京:国家食品安全风险评估中心,2019:122-383.
[5] 杨庆,谭壮生,敬海明,等.基于系统文献检索的副溶血性弧菌危害评估[J].毒理学杂志,2021,35(6):451-454.
[6] 毕红杰,王春东,卢俊荣,等.一起由副溶血性弧菌引起的食源性疾病暴发事件的实验室检测和溯源分析[J].医学动物防制,2019,35(8):800-802.
[7] 毕红杰,张玉梅,任海鹏,等.2016—2017年河北省沧州市食品中食源性致病菌检测结果及分析[J].医学动物防制,2019,35(3):263-266.
[8] 陈佳璇,陈惠玲,刘巧谊,等.2018年广州地区水产品中致病性弧菌的污染调查及其毒力基因检测[J].医学动物防制,2022,38(2):201-204.
[9] 牛蓓,陈磊,罗晓燕,等.2016—2018年河北省食物中毒事件流行特征分析[J].现代预防医学,2020,47(8):1381-1384.
[10] 桂佳,李婵媛,杨赤旗,等.2018年铁岭市食源性致病菌监测结果分析[J].职业与健康,2021,37(2):175-178.
[11] 唐晓阳.水产品中副溶血性弧菌风险评估基础研究[D].上海:上海海洋大学,2013.
[12] 王筱,盛燕华,苏靖华,等.2014—2019年上海市浦东新区食源性致病菌监测分析[J].职业与健康,2020,36(21):2936-2940.
[13] 邹玖明,张爱平,李智山,等.致血流感染非O1群非O139群霍乱弧菌的鉴定及毒力基因检测[J].中国感染与化疗杂志,2014,14(3):186-189.
[14] 申艳琴,兰光,张阳,等.2019年兰州市螺类水产品中致病性弧菌污染状况调查[J].现代食品,2021(5):175-178.
[15] 刘文娟,田云龙,王文君,等.烟台地区水体及水产品中5种致病性弧菌的分布特征及其流行趋势[J].生命科学仪器,2021,19(5):53-57.
[16] LEE K K,LIU P C,CHEN Y C,et al.The implication of ambient temperature with the outbreak of vibriosis in cultured small abalone Haliotis diversicolor supertexta Lischke[J].J Therm Biol,2001,26(6):585-587.
[17] CHANG C C,YEH M S,LIN H K,et al.The effect of Vibrio alginolyticus infection on caspase-3 expression and activity in white shrimp Litopenaeus vannamei[J].Fish Shellfish Immunol,2008,25(5):672-678.
[18] 郭玉坤,朱遇安,范晓明,等.创伤弧菌致病机制的研究进展[J].中华损伤与修复杂志(电子版),2022,17(1):81-84.
[19] 王克波,赵金山,刘丹茹,等.2014—2016年山东省淡水产品中致病性弧菌的污染状况[J].现代预防医学,2017,44(16):2924-2927.
[20] 陈鸿鹄,张云怡,占利,等.2014年浙江省淡水动物性水产品中致病性弧菌污染来源分析[J].中国卫生检验杂志,2018,28(15):1902-1906.
[21] 谭维维,安娜,孙华闽.2017—2020年南通市食源性致病菌监测结果分析[J].公共卫生与预防医学,2022,33(1):71-74.
[22] 李欣,吴健灏,乔雪飞,等.水产品养殖销售环节致病性弧菌污染状况和病原特征分析[J].中国卫生检验杂志,2020,30(17):2069-2073.
[23] 姚利利,沈先标,何平,等.2018年上海市宝山区市售水产品中致病性弧菌污染状况[J].职业与健康,2019,35(15):2060-2063.
[24] 秦政,胡锦瑞,吴科明,等.我国东部沿海地区江河入海口水体中致病性弧菌分布特征[J].中国热带医学,2021,21(6):501-504.
[25] 毕红杰,张玉梅.2017年沧州市食品微生物及其致病因子风险监测结果[J].职业与健康,2018,34(14):1917-1920.