Expression of long noncoding RNA CUST-49525 and CUST-40243 in K562 cytotoxicity induced by 1，4-benzoquinone

Abstract

Abstract: Objective To detect the expression changes of LncRNA CUST-49525 and CUST-40243 in the process of cytotoxicity induced by 1，4-benzoquinone，provide the basis for the later study of their regulatory role in cytotoxicity. Methods K562 cells were exposed to 0，10，20 and 40 μmol/L 1，4-benzoquinone for 24 hours，and the morphological characteristics of the cells were observed under optical microscope. CCK-8 method was used to measure the cell growth. Cell cycle distribution and apoptosis were analyzed by flow cytometry. The expression of LncRNA CUST-49525 and CUST-40243 was measured by real-time fluorescence quantitative PCR. Results After K562 cells were exposed to 1，4-benzoquinone for 24 hours，the number and morphology of K562 cells were obviously changed under microscope. Compared with the control group，the relative proliferation rate of K562 cells in 20 and 40 μmol/L dosage groups decreased significantly（P<0.01）. 1，4-benzoquinone in each dose group could induce cell apoptosis in a concentration-dependent manner（P<0.01）. Compared with the control group，the G1 phase proportion of K562 cells in the 20 and 40 μmol/L dosage groups increased（P<0.01），while the S phase proportion decreased（P<0.05）. The results of real-time quantitative PCR showed that compared with the control group，the expression levels of LncRNA CUST-49525 and CUST-40243 in K562 cells in 20 and 40 μmol/L dosage groups increased（P<0.01）. Conclusion LncRNA CUST-49525 and CUST-40243 are abnormally expressed in the cytotoxicity of K562 cells induced by 1，4-benzoquinone，and they may be involved in the regulation of cytotoxicity，but their specific mechanism needs further study.

Key words: 1，4-benzoquinone, Cytotoxicity, Long noncoding RNA

CLC Number:

R114

LIU Xiao-dong, HUANG Yun-fei, TIAN Wei. Expression of long noncoding RNA CUST-49525 and CUST-40243 in K562 cytotoxicity induced by 1，4-benzoquinone. [J]OCCUPATION AND HEALTH, 2023, 39(1): 9-14.

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