Effect and mechanism of zinc on learning ability impairment of APP/PS1 transgenic mice induced by prenatal chronic stress

Abstract

Abstract: Objective To study the effect and mechanism of zinc intervention on the learning ability of APP/PS1 transgenic mice with prenatal chronic stress and offspring chronic stress. Methods After prenatal chronic stress,32 APP/PS1 transgenic male rats were selected from their offspring. After normal feeding to the age of six months,they were randomly divided into four groups：high,medium and low dose groups（30.34,15.18,6.1 mg/kg） and blank control group,eight rats in each group.From the age of six months,they were subjected to chronic stress for three months and zinc intervention was added at the same time. After the stress treatment,the Morris water maze test was used to test learning and memory ability,pathology of hippocampal CA3 region of mice was observed by hematoxylin eosin（HE） staining,Aβ_1-40 and Aβ_1-42 in hippocampus were detected by ELISA,and the expression level of BACE1 in hippocampus were detected by Western blot. Results Morris water maze：compared with the blank control group,the incubation period of zinc intervention group was shorter,and the number of crossing platforms was more（all P<0.01）.HE staining：the structure of hippocampal CA3 area in the blank control group was disordered,the cells were necrotic and loose,and the number of nerve cells in the zinc intervention group was large,the state was good,and the structure was clear.ELISA experiment and Western blot experiment：compared with the control group,the expression levels of Aβ_1-40,Aβ_1-42 and BACE1 in all dose groups decreased（all P<0.05）. Conclusion The supplement of certain zinc can reduce the expression levels of Aβ_1-40,Aβ_1-42 in hippocampus by decreasing the production of BACE1,so as to improve the damage of hippocampus and learning and memory ability of APP/PS1 transgenic mice caused by prenatal chronic stress combined with offspring chronic stress.

Key words: Alzheimer's disease, Zinc element, APP/PS1 transgenic mice, Learning ability, Chronic stress

CLC Number:

R-332

SU Tong, SHEN Jing, NING Li, TAO Ning, LI Shulin, WANG Qian. Effect and mechanism of zinc on learning ability impairment of APP/PS1 transgenic mice induced by prenatal chronic stress. [J]OCCUPATION AND HEALTH, 2023, 39(20): 2774-2777.

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