Abstract: Objective To monitor the contamination status of anesthetic in commercially available freshwater fish in Pudong New Area of Shanghai from 2021 to 2022,assess the dietary exposure risk. Methods A total of 57 samples of commercially available freshwater fish were randomly collected from 2021 to 2022.The residues of four anesthetics were detected according to the standard operating procedure of the Manual for China National Food Contamination and Harmful Factors Risk Monitoring.Combined with the food intake data of residents,the dietary exposure risk of anesthetic in commercially available freshwater fish was assessed using the index of food safety. Results The total detection rate of anesthetic residues in commercially available freshwater fish in Pudong New Area was 92.98%,and the over-standard rate was 3.51% from 2021 to 2022.The detection rates of eugenol was the highest（89.47%）.There was statistically significant difference on the detection rates of different anesthetics in freshwater fish（P<0.01）.The average and maximum dietary exposure levels of eugenol from freshwater fish were 1.27 μg/（kg·bw·d） and 30.25 μg/（kg·bw·d） respectively. The maximum index of food safety of eugenol was 0.012 1. Conclusion The risk of anesthetic residues in commercially available freshwater fish in Pudong New Area of Shanghai from 2021 to 2022 is at a safe level on the whole,however,the phenomenon of anesthetic residues is relatively common and must be paid enough attention,so it is recommended that relevant national departments should formulate the standards of anesthetic in aquatic products.

Key words: Freshwater fish, Anesthetic, Residual status, Dietary exposure, Risk assessment