Abstract: Objective To understand the occupational fatigue status and influencing factors of medical workers in neurology departments of tertiary hospitals in Suzhou City，analyze its relationship with work stressor and sleep quality. Methods From January to February 2022，the questionnaires were distributed to 153 medical workers in neurology departments of four tertiary hospitals in Suzhou City using the convenient sampling method. The questionnaire included general information questionnaire，occupational fatigue scale，work stressor scale and Pittsburgh sleep quality index scale，and the data were analyzed. Results The total score of occupational fatigue of 153 medical workers in neurology department was （18.12±4.37）points，the total score of work stressor was （68.49±14.53）points，and the total score of Pittsburgh sleep quality index scale was （7.15±1.84）points. The scores of each dimension and total score of work stressors and sleep quality of medical workers in neurology department were positively correlated with the total score of occupational fatigue（all P<0.05）. The overtime >10 times/month，night shift ≥3 times/month，unqualified results of physical examination，work pressure，and sleep quality were all influencing factors of occupational fatigue of medical workers in neurology department（B=0.591，0.724，0.526，0.813，0.579，all P<0.05）. Conclusions The occupational fatigue status of 153 medical workers in neurology department of tertiary hospitals in Suzhou City is not optimistic. The excessive overtime，frequent night shifts，unqualified results of physical examinations，high work pressure，and poor sleep quality are all factors that affect the occupational fatigue of neurosurgery medical staff. Among them，the greater the work pressure and the worse the sleep quality，the more serious the occupational fatigue.

Key words: Neurology department, Medical workers, Occupational fatigue, Work stressors, Sleep quality