Identification and analysis method for chromosomal aberrations in peripheral blood lymphocytes during occupational health examination of radiation workers

Abstract

Abstract: Objective To explore the methods and practical guidance for identifying chromosomal aberrations in peripheral blood lymphocytes during occupational health examinations for radiation workers,so as to provide reference for peer research. Methods From December 2016 to December 2021,6 658 radiation workers were subjected to peripheral blood trace whole blood culture,automatic cell harvesting equipment,and manual production. The chromosome aberration analysis was performed using a fully automated chromosome karyotype scanning analysis system. Results A total of 500 368 metaphase mitotic phases were analyzed,and 142 bicentric bodies（dic）,10 bicentric rings（r）,261 acentric fragments（f）,and 11 obvious translocations（t） were confirmed after review. Three chromosomal forms that need to be distinguished from dicentric bodies（dic） were 79 chromatid intersections,23 chromosomes in contact with each other,and 18 secondary constrictions. The proportion of the sum of these three numbers to the number of bicentric bodies（dic） was 84.5%. Two types of chromosome morphology that need to be distinguished from acentric fragments（f） early separation of 9 and chromosome fissures of 11,and the sum of which was 7.66% in proportion to acentric fragments（f）. Conclusion The proficient identification of various types of chromosome aberrations and mastery of chromosomal aberration identification methods are important quality control measures for chromosome aberration analysis,and are crucial for evaluating the results of the test subject's project.

Key words: Radiation workers, Occupational health examination, Chromosome aberration, Identification and analysis methods

CLC Number:

R148

XIONG Xiaoyun, JIANG Qiuxia, WANG Wei, XU Guang, MAO Shanci. Identification and analysis method for chromosomal aberrations in peripheral blood lymphocytes during occupational health examination of radiation workers. [J]OCCUPATION AND HEALTH, 2024, 40(3): 330-336.

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