Abstract:

Objective To analyze the effects of different blood collection methods on hematological indexes in Wistar rats. Methods Hematological test results of specific pathogen free（SPF） Wistar rats aged 8 weeks in Tianjin Centers for Disease Control and Prevention were collected. A total of 280 test results were collected，among which 80 blood samples were collected from inner canthal veins，80 blood samples were collected from inner canthal veins after anesthesia，120 blood samples were collected from abdominal aorta after anesthesia，with half male and half female. Results There were statistically significant differences in white blood cell（WBC），red blood cell（RBC），hemoglobin（HGB），platelet（PLT），hematocrit（HCT），and leukocyte count among Wistar rats inner canthal venous blood collection group，anaesthesia inner canthal venous blood collection group and abdominal aorta blood collection group（all P<0.05）.The WBC[（9.56±1.97）×10⁹/L]，RBC[（8.16±0.41）×10¹²/L]，and HGB[（157.07±6.53）g/L] detection values obtained by inner canthal veins after anesthesia were higher than the WBC[（8.12±1.72）×10⁹/L]，RBC[（7.46±0.45）×10¹²/L]，and HGB[（143.38±9.08）g/L] detection values obtained by inner canthal veins，and were also higher than the WBC [（4.21±2.22）×10⁹/L]，RBC[（6.68±0.49）×10¹²/L]，and HGB[（136.18±8.81）g/L] detection values obtained by abdominal aorta puncture after anesthesia. The PLT values in the inner canthal veins[（996.08±171.58）×10⁹/L] were lower than those measured by the inner canthal veins after anesthesia[（1233.89±272.38）×10⁹/L] and the abdominal aorta blood sampling method after anesthesia [（1 172.61±151.16）×10⁹/L]. There was no statistically significant difference（all P>0.05） between the PLT values measured by the conjunctival vein method after anesthesia and the abdominal aorta blood sampling method after anesthesia. Conclusion The WBC，RBC and HGB venous blood values of Wistar rats are higher than arterial blood values，and peritoneal injection of 50 mg/（kg·BW） pentobarbital sodium has a certain effect on the hematological detection.

Key words: Blood collection methods, Wistar rats, Hematological indexes