Abstract:

Objective To establish a high performance liquid chromatography（HPLC） method for the simultaneous determination of abrine，Erythrine and Shaftoside in Abrus cantoniensis Hance，and to conduct correlation analysis on the content determination results of leaves，stems and roots of Abrus cantoniensis Hance，exploring the content characteristics and regularity of different medicinal parts of Abrus cantoniensis Hance. Methods ZORBAX SB-C8 chromatographic column（4.6 mm×250 mm，5 μm） was used，with 0.2% ammonium acetate-triethylamine buffer solution（pH=8.0）-methanol as mobile phase，gradient elution，flow rate of 1.0 mL/min，and detection wavelength of 220，277 nm. Correlation analysis were carried out by using IBM SPSS Statistics 27 statistical software. Results Abrine，Erythrine and Shaftoside had a good linear relationship in the range of 9.15-274.60 μg/mL（r=0.999 7），9.02-270.50 μg/mL（r=0.999 8），18.87-377.40 μg/mL（r=0.999 8），respectively. The recovery rate of each component was in the range of 92.2%-108.0%. Abrine in Abrus cantoniensis Hance stem was positively correlated with Erythrine（P<0.01），while there was no significant correlation between different components in other parts. There were statistically significant differences in the contents of Abrine，Erythrine and Shaftoside and the total amount of the three components in leaves，stems and roots（all P<0.01）. Conclusion The method established in this study for simultaneous determination of the contents of abrine，Erythrine and Shaftoside in Abrus cantoniensis Hance has strong operability and high accuracy，and a reasonable correlation analysis method，providing a scientific basis for quality control and use of Abrus cantoniensis Hance.

Key words: Abrus cantoniensis Hance, Medicinal part, Content determination, Correlation analysis, Abrine, Erythrine, Shaftoside