Effects of 1-nitropyrene on lifespan of Caenorhabditis elegans through autophagy pathway

Abstract

Abstract: Objective By using the Caenorhabditis elegans model（hereafter referred to as C. elegans）,to investigate the effects of 1-nitropyrene（1-NP） on the lifespan of C. elegans and the role of autophagy in the impact of lifespan. Methods The effects of subacute exposure（five days） of 1-NP（0,10^-4,10^-2,10²,10⁴ ng/L） on the life span of C. elegans were studied under normal culture conditions. The effects of 1-NP on health indicators（body length,exercise capacity and lipofuscin） and longevity of C. elegans were observed. The expression of autophagy related marker genes（bec-1 and lgg-1） was detected by quantitative real time-polymerase chain reaction（qRT-PCR）. Results Compared with the control group,1-NP could significantly reduce the lifespan of C. elegans,and 10⁴ ng/L group had the most significant effect,with a reduction rate of 28.2%. 1-NP（10⁴ ng/L） decreased the body length of C. elegans（P<0.05） and the expression levels of key autophagy genes（bec-1 and lgg-1）（P<0.05）,increased lipofuscin accumulation in C. elegans（P<0.05）. Conclusion 1-NP can shorten the lifespan of C. elegans by decreasing the autophagy level in C. elegans.

Key words: 1-nitropyrene, Caenorhabditis elegans, Aging, Autophagy, Lipofuscin

CLC Number:

R114

FU Yucong, WANG Meng, LIU Shengjie, ZHAO Lile, ZHANG Xumei, ZHANG Liwen. Effects of 1-nitropyrene on lifespan of Caenorhabditis elegans through autophagy pathway. [J]OCCUPATION AND HEALTH, 2024, 40(2): 195-199.

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