Simultaneous determination of acrylamide and L-asparagine in Atractylodis macrocephalae rhizoma after thermal processing by multi-walled carbon nanotubes-dispersive solid-phase extraction combined with liquid chromatography-tandem mass spectrometry

Abstract

Abstract: Objective To establish a method for simultaneous determination of acrylamide andL-asparagine in Atractylodis macrocephalae rhizome（AMR）,analyze the content change of acrylamide and L-asparagine during the thermal processing of AMR,so as to providebasis for the research of processing technology. Methods The simultaneous determination of L-asparagine and acrylamide in AMR was realized by dispersed solid phase extraction using multi-walled carbon nanotubes（MWCNTs） and liquid chromatography tandem mass spectrometry（LC-MS/MS）.Using this method,the contents of L-asparagine andacrylamide in processed AMR samples were determined under different processing temperature and time. The risk of acrylamide contamination in AMR was evaluated by margin of exposure（MOE）. Results In the validation of the optimized method,the recoveries of L-asparagine were 98.4%-103.2% with relative standard deviation（RSD） of 2.5%-3.6%. The recoveries of acrylamide were 97.5%-101.9% with RSD of 1.9%-3.1%. The linear relationships of acrylamide and L-asparagine were good within the range of 1-50 ng/mL and 25-1000 ng/mL respectively,and the correlation coefficients were ≥0.99. The detection limit of acrylamide and L-asparagine was 0.003 mg/kg and 0.1 mg/kg respectively. During the processing of AMR,L-asparagine showed a downward trend with the increase of time,and the content of acrylamide first increased and then decreased with the processing time. According to the change of acrylamide content in AMR after processing,the lowest value was 79.3 μg/kg and the highest value was 1558.9 μg/kg,which led to the lowest MOE value of 673.6,indicating safety risk. Conclusions The study establishes and verifies a method for the simultaneous determination of acrylamide and L-asparagine. After the thermal processing of AMR,the concentration of acrylamide increases significantly,resulting in potential safety risks. Comprehensive consideration was needed to further improve and standardize the processing technology.

Key words: Acrylamide, L-asparagine, Atractylodis macrocephalae rhizoma, Thermal processing, Margin of exposure

CLC Number:

R115

XU Xiao-ying, LIANG Jing-jing, ZHU Bing-qi, LANG Wen-jun, LIU Zhu. Simultaneous determination of acrylamide and L-asparagine in Atractylodis macrocephalae rhizoma after thermal processing by multi-walled carbon nanotubes-dispersive solid-phase extraction combined with liquid chromatography-tandem mass spectrometry. [J]OCCUPATION AND HEALTH, 2023, 39(10): 1306-1311.

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