Research progress of rapid detection techniques of mycotoxin-producing fungi in food

Abstract

Abstract: The most common mycotoxin-producing fungi in food are Aspergillus,Penicillium,Fusarium,Trichoderma,Cephalosporium and Trichothecium,etc. The mycotoxins produced by these mycotoxin-producing fungi pose serious harms to human and animal health,and also cause huge economic losses to agriculture. Direct detection of mycotoxin-producing fungi,rather than mycotoxins,can be more prospective to reduce the harm of mycotoxin-producing fungi to humans and animals. This article reviews the current rapid detection methods directly targeting toxigenic mold,including immunological methods,nucleic acid detection methods and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry detection methods,in order to provide reference for the development of more rapid and accurate detection methods for mycotoxin-producing fungi.

Key words: Mycotoxin-producing fungi, Rapid detection, Polymerase chain reaction, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

CLC Number:

R115

GAO Jiacong, WANG Yun, YUE Mingxiang. Research progress of rapid detection techniques of mycotoxin-producing fungi in food. [J]OCCUPATION AND HEALTH, 2024, 40(7): 988-991.

References

[1] MORETTI A,SUSCA A,MULÉ G,et al.Molecular biodiversity of mycotoxigenic fungi that threaten food safety[J].Int J Food Microbiol,2013, 167(1):57-66.
[2] ALSHANNAQ A,YU J H.Occurrence,Toxicity,andAnalysis of Major Mycotoxins in Food[J].Int J Environ Res Public Health,2017,14(6):632.
[3] MARTÍNEZ-CULEBRAS P V,GANDÍA M,BORONAT A,et al.Differential susceptibility of mycotoxin-producing fungi to distinct antifungal proteins (AFPs)[J].Food Microbiol,2021,97:103760.
[4] PLEADIN J,FRECE J,MARKOV K.Mycotoxins in food and feed[J].Adv Food Nutr Res,2019,89:297-345.
[5] WU F.Mycotoxin risks are lower in biotech corn[J].Curr Opin Biotechnol,2022,78:102792.
[6] HSU M C,CHEN K W,LO H J,et al.Species identification of medically important fungi by use of real-time Light Cycler PCR[J].J Med Microbiol,2003,52(Pt 12):1071-1076.
[7] SCHAMANN A,SCHMIDT-HEYDT M,GEISEN R.Analysis of the competitiveness between a non-aflatoxigenic and an aflatoxigenic Aspergillus flavus strain on maize kernels by droplet digital PCR[J].Mycotoxin Res,2022,38(1):27-36.
[8] SHEKHAR M,SINGH N,DUTTA R,et al.Comparative study of qualitative and quantitative methods to determine toxicity level of Aspergillus flavus isolates in maize[J].PLoS One,2017,12(12):e0189760.
[9] WIGMANN É F,BEHR J,VOGEL R F,et al.MALDI-TOF MS fingerprinting for identification and differentiation of species within the Fusarium fujikuroi species complex[J].Appl Microbiol Biotechnol,2019, 103(13):5323-5337.
[10] LI S,MARQUARDT R R,ABRAMSON D.Immunochemical detection of molds:a review[J].J Food Prot,2000,63(2):281-291.
[11] WANG T,LI P,ZHANG Q,et al.Determination of Aspergillus pathogens in agricultural products by a specific nanobody-polyclonal antibody sandwich ELISA[J].Sci Rep,2017,7(1):4348.
[12] KUMARI R,GHOSH A K.Detection of aspergillus flavus in wheat grains using anti-mannoprotein(mp1) and spore protein polyclonal antibodies[J].Appl Biochem Biotechnol,2022,194(4):1790-1803.
[13] MANONMANI H K,ANAND S,CHANDRASHEKAR A,et al.Detection of aflatoxigenic fungi in selected food commodities by PCR[J].Process Biochem,2005,40:2859-2864.
[14] SUSCA A,STEA G,MULÈ G,et al.Polymerase chain reaction(PCR) identification of Aspergillus niger and Aspergillus tubingensis based on the calmodulin gene[J].Food Addit Contam,2007,24(10):1154-1160.
[15] RAHMAN H U,YUE X,REN X,et al.Multiplex PCR assay to detect aspergillus,penicillium and fusarium species simultaneously[J].Food Addit Contam Part A Chem Anal Control Expo Risk Assess,2020, 37(11):1939-1950.
[16] DEGOLA F,BERNI E,DALL'ASTA C,et al.A multiplex RT-PCR approach to detect aflatoxigenic strains of Aspergillus flavus[J].J Appl Microbiol,2007,103(2):409-417.
[17] RAHMAN U H.农产品中产毒真菌及真菌毒素同步检测技术研究[D].北京:中国农业科学院,2020.
[18] HARSHITHA R,ARUNRAJ D R.Real-time quantitative PCR:A tool for absolute and relative quantification[J].Biochem Mol Biol Educ,2021,49(5):800-812.
[19] WANG S,CUI H,CHEN M,et al.Quantitative PCR assays for the species-specific detection of Fusarium graminearum sensu stricto and Fusarium asiaticum in winter wheat growing regions in China[J].Int J Food Microbiol,2023,387:110061.
[20] ABD EL-AZIZ A R M,SHEHATA S M,HISHAM S M,et al.Molecular profile of aflatoxigenic and non-aflatoxigenic isolates of Aspergillus flavus isolated from stored maize[J].Saudi J Biol Sci,2021,28(2):1383-1391.
[21] 张庆,赵晓美,陈颖,等.产毒真菌多重实时荧光PCR快速检测方法建立[J].卫生研究,2020,49(6):881-888.
[22] LEI S,CHEN S,ZHONG Q.Digital PCR for accurate quantification of pathogens:Principles,applications,challenges and future prospects[J].Int J Biol Macromol,2021,184:750-759.
[23] WANG D,WANG S,DU X,et al.ddPCR surpasses classical qPCR technology in quantitating bacteria and fungi in the environment[J].Mol Ecol Resour,2022,22(7):2587-2598.
[24] TSUCHIDA S,UMEMURA H,NAKAYAMA T.Current status of Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry(MALDI-TOF MS) in clinical diagnostic microbiology[J].Molecules,2020,25(20):4775.
[25] 邵锦,万喆,李若瑜,等.基质辅助激光解析电离飞行时间质谱在医学真菌领域的应用进展[J].菌物学报,2019,38(8):1277-1286.
[26] SENDID B,DUCOROY P,FRANÇOIS N,et al.Evaluation of MALDI-TOF mass spectrometry for the identification of medically-important yeasts in the clinical laboratories of Dijon and Lille hospitals[J].Med Mycol,2013,51(1):25-32.
[27] QUÉRO L,GIRARD V,PAWTOWSKI A,et al.Development and application of MALDI-TOF MS for identification of food spoilage fungi[J].Food Microbiol,2019,81:76-88.
[28] SLEIMAN S,HALLIDAY C L,CHAPMAN B,et al.Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of aspergillus,scedosporium,and fusarium spp.in the australian clinical setting[J].J Clin Microbiol,2016,54(8):2182-2186.
[29] SINGH A,SINGH P K,KUMAR A,et al.Molecular and matrix-assisted laser desorption ionization-time of flight mass spectrometry-based characterization of clinically significant melanized fungi in India[J]. J Clin Microbiol,2017,55(4):1090-1103.
[30] HLEBA L,HLEBOVA M,KOVACIK A,et al.Use of MALDI-TOF MS to discriminate between aflatoxin b1-producing and non-producing strains of aspergillus flavus[J].Molecules,2022,27(22):7861.
[31] QUÉRO L,COURAULT P,CELLIÈRE B,et al.Application of MALDI-TOF MS to species complex differentiation and strain typing of food related fungi:Case studies with aspergillus section flavi species and penicillium roqueforti isolates[J].Food Microbiol,2020,86:103311.
[32] HEDAYATI M T,TAGHIZADEH-ARMAKI M,ZARRINFAR H,et al.Discrimination of Aspergillus flavus from Aspergillus oryzae by matrix-assisted laser desorption/ionisation time-of-flight(MALDI-TOF) mass spectrometry[J].Mycoses,2019,62(12):1182-1188.