Correlation between IQUB and swimming exercise in inhibiting tumor growth in nude mice with hepatocellular carcinomabased on RNA-seq

Abstract

Abstract: Objective To investigate the molecular mechanism of swimming exercise in inhibiting tumor growth in nude mice with hepatocellular carcinoma（HCC） based onRNA-Sequence（RNA-seq）. Methods Ten 4-week-old BALB/c male nude mice were randomly divided into swimming group and control group. The nude mice in the swimming group received subcutaneous tumor inoculation for 1 week after swimming acclimation,and then received swimming training for 4 weeks（0 weight bearing,30 min/d, 5 days/week,consecutive four weeks）. After 4 weeks of swimming training,nude mice were sacrificed. Serum was collected and tumor tissues were measured.RNA-seq was used to analyze the related intervention mechanism. The real-time reverse transcription polymerase chain reaction assay（RT-qPCR） was used to verify the effect of swimming exercise on IQ motif and ubiquitin domain containing（IQUB） mRNA expression in HCC tissues of nude mice. Based on hepatocellular carcinoma cell line SMMC-7721,the effects of nude mouse serum on proliferation,Wnt/β-catenin signaling pathway and IQUB mRNA expression of SMMC-7721. Results The average tumor weight of exercise group was （0.136±0.020）g,which was significantly decreased compared with control group[（0.290±0.099）g]（P<0.05）. The results of RNA-Seq showed that the differential gene IQUB may play an important role in the anti-HCC effect of swimming,and it is closely related to the Wnt/β-catenin signaling pathway.Compared with the control group,the relative expression level of IQUB in the exercise group was 0.23±0.05（P<0.05）. Compared with the control group,the relative expression level of IQUB mRNA in exercise serum intervention group was 0.22±0.03（P<0.05）. The serum of nude mice in exercise group could inhibit the activation of Wnt/β-catenin signaling pathway of HCC cells by LiCl. The expression trend of IQUB in HCC cells was consistent with the protein expression of Wnt/β-catenin signaling pathway under the co-intervention of nude mouse serum and LiCl. Conclusion The swimming exercise can inhibit tumor growth in nude mice model of HCC,and IQUB may be an upstream regulator of Wnt/β-catenin signaling pathway,and may be a potential target of swimming exercise to inhibit the development of HCC.

Key words: Aerobic exercise, Swimming, RNA-seq, Hepatocellular carcinoma, IQUB, Wnt/β-catenin pathway

CLC Number:

R-332

XIAO Chulan, CHEN Jiaojiao, YIN Zifei, LI Bai. Correlation between IQUB and swimming exercise in inhibiting tumor growth in nude mice with hepatocellular carcinomabased on RNA-seq. [J]OCCUPATION AND HEALTH, 2023, 39(19): 2617-2625.

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