Analysis of virulence genes and drug resistance of Vibrio parahaemolyticus in aquatic products and aquaculture water in Shenzhen City from 2015-2020

Abstract

Abstract: Objective To understand the virulence genes and drug resistance spectrum of Vibrio parahaemolyticus in aquatic products and aquaculture water in Shenzhen City from 2015 to 2020. Methods The virulence genes of thermostable direct hemolysin（TDH）,TDH-related hemolysin（TRH） and thermolabile hemolysin（TLH） of 96 strains of Vibrio parahaemolyticus from the food risk and cholera surveillance in Shenzhen City were screened by multiplex fluorescence quantitative PCR technology. The resistance to 17 kinds of antibiotics was determined by the minimum inhibitory concentration（MIC）. Results None of the 96 strains of Vibrio parahaemolyticus contained tdh and trh genes,and the tlh gene carrier rate was 100%. The resistance rate of strains to ampicillin was the highest,which was 93.75%,and the intermediate rate was 5.21%. The intermediate rate to cefazolin was the highest,which was 42.71%,followed by cefepime and aztreonam,the intermediary rates were 16.67% and 10.42% respectively. All strains were sensitive to nine antibiotics including cefotetan,ceftazidime,ciprofloxacin,gentamicin,ceftriaxone,nitrofurantoin,imipenem,levofloxacin and tobramycin,and the sensitivity rate was 100%. Conclusion The virulence of Vibrio parahaemolyticus from aquatic products and aquaculture water in Shenzhen City from 2015 to 2020 is less,it is generally resistant to ampicillin,the strains also have a certain degree of resistance and intermediary to other antibiotics,and individual strains show multidrug resistance.

Key words: Vibrio parahaemolyticus, Drug resistance, Aquatic products, Minimum inhibitory concentration, Antibiotics

CLC Number:

R117

ZHANG Jinjin, CHEN Haochuan, CHEN Runli, WANG Jiahao, LI Lingling, LIU Song. Analysis of virulence genes and drug resistance of Vibrio parahaemolyticus in aquatic products and aquaculture water in Shenzhen City from 2015-2020. [J]OCCUPATION AND HEALTH, 2023, 39(21): 3011-3014.

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