Abstract: Objective To analyze the acute toxicity and genotoxicity of 1-ethynylcyclohexanol,and provide toxicological data for its safety use. Methods The up and down procedure（UDP） was used to determine the acute oral toxicity of 1-ethynylcyclohexanol. The toxicity of 1-ethynylcyclohexanol to cells in vitro was evaluated by observing the changes in cell morphology and cell survival rate after exposure. The genotoxicity of 1-ethynylcyclohexanol was detected using mouse bone marrow micronucleus assay,salmonella typhimurium reverse mutation assay（Ames test）,and in vitro chromosomal aberration assay based on double chamber slides. Results The median lethal dose（LD₅₀） of acute oral toxicity of 1-ethynylcyclohexanol in mice was 1 098 mg/kg·bw（95%CI 550-2 000 mg/kg·bw）.As the dose of 1-ethynylcyclohexanol increases,it was observed under the microscope that the adherent cell morphology gradually became round until most of the cells were autolysed,and the number of cells gradually decreases. The tetramethylazolyl blue（MTT） assay showed that the semi-inhibitory concentration（IC₅₀） of 1-ethynylcyclohexanol on Chinese hamster lung cells（CHL） were 1 970（+S9,6 h）、1 935（-S9,6 h）和1 835（-S9,24 h）μg/mL.Ames test results indicated that under ± S9 conditions,each dose group had no mutagenic effect on the four test strains（TA979a、TA98、TA100、TA102）. The average rates of micronucleus containing cells in male and female mice in the three dose groups had no statistically significant difference compared with the negative control group（all P>0.05）. The results of the in vitro chromosome aberration test showed that under the three treatment methods of ±S9（6 h） and -S9（24 h）,there were no statistical significance in chromosome aberration rates of each dose compared with negative control group（all P>0.05）. Conclusion Under the conditions of this experiment,the acute oral toxicity of 1-ethynylcyclohexanol is classified as low toxicity. It exhibits cytotoxicity at concentrations of 500-2 500 μg/mL and is positively correlated with dose,but no genotoxicity was observed.

Key words: 1-ethynylcyclohexanol, Acute oral toxicity, Cytotoxicity, Genotoxicity