Abstract: Objective To establish a determination method by inductively coupled plasma-mass spectrometry（ICP-MS） for 20 elements in blood which was direct diluted with matrix modifier,and apply in the detection of the general population,occupational population,and poisoned population. Methods The blood samples were diluted 20-fold with 0.50% nitric acid（HNO₃）-0.10% Triton X-100. The mixed normal human blood was added with different concentrations of mixed standard solution to prepare standard sequences. The internal standard solution of yttrium（Y） and lutetium（Lu） at the concentration of 10 μg/L was simultaneously injected.ICP-MS with standard（STD） and kinetic energy discrimination（KED） mode was used to determine the content of the samples by internal standard correction method. Results In the linear range of 0.100-100.000 μg/L,the linear relationship of the 20 elements was ≥0.999. The limit of detection was 0.001-0.096 μg/L and the lower limit of quantitation was 0.003-0.320 μg/L. The relative standard deviation（RSD） within and between batches was 0.4%-4.6% and 1.5%-4.9%,respectively.The recovery rate was 80.9%-111.2%. Conclusion This method is easy to operate,fast,with high recovery rate,good reproducibility and strong practicality,it is suitable for the detection of 20 elements in blood samples of the general population,occupational population and poisoned population,providing a new method for blood metabolomics analysis.

Key words: Matrix modifier, Direct dilution method, Inductively coupled plasma-mass spectrometry, Blood sample, Element