桑辛素通过 Wnt/β-catenin 信号通路抑制胶质瘤细胞的机制研究

doi:10.13329/j.cnki.zyyjk.2021.0007

职业与健康 ›› 2021, Vol. 37 ›› Issue (1): 33-36.DOI: 10.13329/j.cnki.zyyjk.2021.0007

桑辛素通过 Wnt/β-catenin 信号通路抑制胶质瘤细胞的机制研究

十堰市太和医院 a 神经内科，b 神经外科三病区，湖北十堰 442000

收稿日期:2020-05-27 接受日期:2020-08-24 出版日期:2021-01-01 发布日期:2021-03-03
通讯作者: 陈俊，主任医师，教授，E-mail：cjunthyy@protonmail.com
作者简介:杨璐，男，医师，主要从事胶质瘤、急性缺血性脑血管病、中枢神经脱髓鞘疾病、睡眠障碍、脊髓病等神经内科疾病研究工作。
基金资助:
湖北医药学院中青年人才项目（Q20192103）

Study on mechanism of morusin inhibiting glioma cells through Wnt/β-catenin signaling pathway

a Department of Neurology， b The third ward of Neurosurgery Department， Taihe Hospital in Shiyan， Shiyan Hubei， 442000，China

Received:2020-05-27 Accepted:2020-08-24 Online:2021-01-01 Published:2021-03-03

摘要/Abstract

摘要：

目的探讨桑辛素在胶质瘤细胞的抗癌作用。方法用 CCK-8 检测细胞增殖情况，利用 Traswell 试验进行细胞迁移分析，用异硫氰酸荧光素（Annexin V-FITC）凋亡检测试剂盒检测细胞凋亡情况，采用免疫印迹试验（Western blotting）和实时荧光定量 PCR（RT-qPCR）检测靶基因 mRNA 和蛋白水平的变化。结果桑辛素能明显抑制 U251 细胞的增殖活性（P＜0.05），其中 100 和 1 000 μg/mL 桑辛素的抑制效果最佳。 100 μg/mL 的桑辛素能明显抑制 U251 细胞的迁移能力（P＜0.05），并诱导细胞凋亡（P＜0.05）。 Wnt/β-catenin 信号通路参与桑辛素诱导的抗肿瘤活性，桑辛素可下调 Wnt/β-catenin 信号通路相关蛋白（β-catenin 和 Cyclin D1）的表达（P＜0.05）。结论桑辛素可通过 Wnt/β-catenin 信号途径抑制胶质瘤细胞的生长，

因此，桑辛素可能是一种潜在的胶质瘤抗癌药物。

关键词:

桑辛素, Wnt/β-catenin 信号通路, 胶质瘤

Abstract:

Objective To investigate the anticancer effect of moristin on glioma cells. Methods Cells proliferation was examined by CCK-8. Migration assays were performed using transwell assay. Apoptotic cells were detected using the Annexin V-FITC apoptosis detection kit. Western blotting and real time fluorescence quantitative PCR（RT-qPCR） were used to exam the change of target gene in mRNA and protein level. Results Morusin can obviously inhibited the proliferation activity of U251 cells（P<0.05）， of which 100 and 1000 μg/mL had the best inhibitory effect. 100 μg/mL of morusin can obviously inhibited the migration ability of U251 cells and induce cell apoptosis（both P<0.05）. Wnt/β-catenin signaling pathway was involved in anti-tumor activity induced by moristin， and morusin can down-regulate the expression of Wnt/β-catenin signaling pathway-related proteins （β-catenin and Cyclin D1（P<0.05）. Conclusion Morusin can inhibit the growth of glioma cells through Wnt/β-catenin signal pathway. Thus，morusin could be a potential anti-cancer agent for glioma.

Key words:

Morusin, Wnt/β-catenin signal pathway, Glioma

杨璐, 周一, 鲍毅, 孙强, 刘光健, 陈俊.

桑辛素通过 Wnt/β-catenin 信号通路抑制胶质瘤细胞的机制研究

[J]. 职业与健康, 2021, 37(1): 33-36.

YANG Lu, ZHOU Yi, BAO Yi, SUN Qiang, LIU Guang-jian, CHEN Jun.

Study on mechanism of morusin inhibiting glioma cells through Wnt/β-catenin signaling pathway

[J]. OCCUPATION AND HEALTH, 2021, 37(1): 33-36.

桑辛素通过 Wnt/β-catenin 信号通路抑制胶质瘤细胞的机制研究

Study on mechanism of morusin inhibiting glioma cells through Wnt/β-catenin signaling pathway

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