Effects of BDE-47 exposure during pregnancy on adipokines and insulin sensitivity in mice

Abstract

Abstract: Objective To preliminarily investigate the effects of 2,2',4,4'-tetrabromodiphenyl ether（BDE-47） exposure during pregnancy on adipokines and insulin sensitivity in maternal and offspring mice,explore its possible mechanisms. Methods The female C57BL/6 mice were randomly divided into high,medium,low dose groups and control group,five animals in each group. After being fed for one week,they were caged with healthy adult male mice purchased in the same batch. The female mice were given BDE-47 by intragastric administration for 10 days after successful cage closure. One week after gavage,the maternal mice were treated with glucose tolerance test by intraperitoneal injection. At six weeks after birth,all mice fasted overnight and were killed. The levels of serum insulin,leptin and adiponectin in maternal mice were measured by enzyme-linked immuno sorbent assay（ELISA）. The mRNA and protein expression levels of peroxisome proliferator-activated receptor γ（PPARγ） leptin and adiponectin in the liver tissues of maternal and offspring mice were detected by quantitative real-time PCR（qRT-PCR） and Western blot. Results BDE-47 exposure caused a disturbance of glucose metabolism in maternal mice. The body mass of the offspring mice increased during the early development period after birth,and the liver weight and organ coefficients of the maternal mice and male offspring in the exposure groups were higher than those in the control group（all P<0.05）. Compared with the control group,the mRNA and protein expression of PPARγ in the liver tissue of maternal mice and female offspring in the highdose group decreased,and the mRNA and protein expression of PPARγ in the liver tissue of male offspring in the high dose group increased（all P<0.05）. The mRNA and protein expression of leptin in maternal mice in the high and medium dose groups were lower than those of the control group,the mRNA expression of leptin in female offspring in the high dose group was higher than that of other groups,and the mRNA expression of leptin in the liver tissue of male offsring in the exposure groups was lower than that of the control group（all P<0.05）. The mRNA expression of adiponectin in maternal mice and female offspring in the medium dose group were lower than that of the control group,and the mRNA expression of adiponectin in the liver tissue of male offspring in each dose group were lower than that of the control group（all P<0.05）. Compared with female offspring,the liver organ coefficients of male offspring in the high dose group increased,the mRNA and protein expression of PPARγ increased,the protein expression of leptin in male offspring mice in the medium and high dose groups was higher than that in female offspring mice,and the mRNA expression of adiponectin in male offspring mice in the low and high dose groups was lower than that female offspring mice（all P<0.05）. Conclusion BDE-47 causes glucose metabolism disorder in maternal mice,increases body weight during early development of offspring mice,and affects the expression of PPARγ,leptin and adiponectin genes in mice,and there are gender differences.

Key words: 2,2',4,4'-tetrabromodiphenyl ether, Birth outcome, Adipokines, PPAR γ, Sex difference

CLC Number:

R-332

JIANG Shu-rui, WANG Jia-sui, WANG Yong-zhi, WANG Meng-lin, Zunire·Tuerxun, LIU Zao-ling. Effects of BDE-47 exposure during pregnancy on adipokines and insulin sensitivity in mice. [J]OCCUPATION AND HEALTH, 2023, 39(12): 1589-1595.

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