Analysis on genotyping of foodborne Listeria monocytogenes in commercially available food of Shanghai in 2020

Abstract

Abstract: Objective To study the genotyping characteristics of Listeria monocytogenes（LM） in commercially available food of Shanghai in 2020. Methods Totally 62 strains in 2020 were analyzed by pulsed-field gel electrophoreisi（PFGE） and whole genome sequencing（WGS）. Results PFGE clustering analysis of 62 strains of LM identified 17 types and the dominant band was type 1. The multilocus sequence typing（MLST） of WGS data showed 12 sequence type（ST） types and the dominant type was ST9. All carried fosfomycin antibiotic gene（fosX）,listeria pathogenicity island-1（LIPI-1）,listeria pathogenicity island-2（LIPI-2）,listeria pathogenicity island-3（LIPI-3） and other virulence gene. Some strains carried aminoglycoside antibiotic gene[ANT（6）-la],macrolide antibiotic gene（ErmB）,trimethoprim resistant gene（dfrG）,tetracycline resistant gene（tetM）,lincosamide antibiotic gene（lin）,and chloramphenicol antibiotic gene（cat）. There were strains resistant to tetracycline,chloramphenicol and erythromycin,with serotypes of 1/2a,1/2b,1/2c and 4b. Conclusion The molecular typing of foodborne Listeria monocytogenes in commercially available food of Shanghai in 2020 has high diversity,there are many pathogenic types,the comparison effect of homology is MLST>PFGE> serotype,carring a variety of virulence genes,and ome strains show drug resistance characteristics.

Key words: Listeria monocytogenes, Plused-field gel electrophoreisi, Whole genome sequencing, Drug resistance

CLC Number:

R155.5

WANG Xiao, DING Jin, CUI Qiqi, ZHOU Yuqing, PAN Lifeng, HAO Lipeng. Analysis on genotyping of foodborne Listeria monocytogenes in commercially available food of Shanghai in 2020. [J]OCCUPATION AND HEALTH, 2023, 39(13): 1774-1778.

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