Abstract: Objective To establish a novel, rapid, and accurate ulra-high performanee liquid chromatography tandem triplequadrupole mass speclrometry（LC-MS/MS） detection method for the simultaneous detection of 11 β₂-receptor agonists（tobuterol, marbuterol, fenoterol, zilpaterol, pentbuterol, brobuterol, bambuterol, salbutamol, ractopamine, terbutaline, clenbuterol） and 4 β-recepltor blockers（propranolol, natolol, carazololum, atenolol） in meat products. Methods The meal products were extracted by 5% trichloroacetic acid（TCA）, purilied on a Waters Oasis MCX column, and the mobile phases were 0. 1% formic acid water +10 mmol/L ammonium acetate. The sample were separated by C₁₈ column by gradient elution method, and quantitated by muliplereaction detection mode. Results The linear relationship of 11 β₂-receptor agonists and 4 β-receptor blockers showed good stability at 0. 5-20 mg/L, with a correlation coefficient r>0. 999 0. The spiked recovery rate of the method was 58. 5%-113. 2%, and the relative standard deviation was 0. 3%-4. 9%. Conclusion The established method is highly accurate, sensitive and suitable for the detection of 11 β₂-receptor agonists and 4 β-receptor blockers in meat products.

Key words: β₂-receptor agonists, β-receptor blockers, Ulura-high performance liquid chromatography tandem triple quadrupolemass spectrmetry, Multi component residue