Abstract: Objective To establish a liquid chromatography tandem mass spectrometry-multiple reaction monitoring-information dependent acquisition-enhanced product ion（LC-MS/MS-IDA-EPI） method for simultaneous determination of 16 indole alkaloids in Chinese herbal medicine and poisoning samples,including gelsemium elegans alkaloids,evodia rutaecarpa alkaloids,U. rhynchophylla alkaloids,yohimbine,strychnine,etc. Methods The 16 indole alkaloids were separated on a Phenomenex Kinetex C₁₈ column using mobile phases of 0.01% ammonia（v/v） aqueous solution and methanol for gradient elution at a flow rate of 0.4 mL/min. The multiple reaction monitoring-information dependent acquisition-enhanced product ion（MRM-IDA-EPI） scanning modes was used for detection. Results The matching degree of the EPI spectra for the positive samples was greater than 70%,with a deviation in retention time within ±0.1 min. All compounds had good linear relationships within the corresponding concentration range,with correlation coefficients >0.998. The limits of detection and the limit of quantification were 0.01-4.00（μg/L or μg/kg） and 0.04-13.00（μg/L or μg/kg）,respectively. The average recovery rate was 83.43% to 109.41%,and the relative standard deviations（RSD） were 1.70% to 4.51%（n=6）. Conclusion The established method is simple,rapid,accurate,and reliable,and can be used for the rapid qualitative and quantitative analysis of 16 indole alkaloids in Chinese herbal medicine and poisoning samples.

Key words: Indole alkaloids, Liquid chromatography tandem mass spectrometry, Multiple reaction monitoring-information dependent acquisition-enhanced product ion scan, Chinese herbal medicine, Poisoning samples