Abstract: Objective To establish efficient and convenient detection methods for detecting 11 kinds of quinolones,4 kinds of tetracycline and chloramphenicol residues in chicken liver and gizzard. Methods After processing chicken gizzards and liver through extraction,purification and solid phase extraction,UPLC-MS/MS was used to separate and quantitatively determine the residues of veterinary drugs. Results The linear range of residues of 16 veterinary drugs was 1.0-40 ng/mL（chloramphenicol 0.1-4.0 ng/mL）,the correlation coefficient was greater than 0.999 0 except norfloxacin,and the average recovery rate was 61.6%-107.1% determined at different concentrations,and the detection limit of norfloxacin, ciprofloxacin, dafloxacin, tetracycline, doxycycline, oxytetracycline, chlortetracycline, doxycycline, and chlortetracycline was 0.6 μg/kg, and the limit of quantification was 1.8 μg/kg. The detection limit of difloxacin, pefloxacin, ofloxacin, enrofloxacin, lomefloxacin, salafloxacin, oxaquinic acid, and flumequine was 0.2 μg/kg, and the limit of quantification was 0.6 μg/kg. The detection limit of chloramphenicol was 0.02 μg/kg, and the limit of quantification was 0.06 μg/kg. Conclusion Using this method to detect the chicken gizzards and liver samples collected in 2022,the liner range, detection limit, precision and accuracy all meet the detection requirements. This method improves the detection efficiency,is suitable for veterinary drug screening and detection of a large number of samples of chicken liver and gizzard on the market,and is suitable as a rapid screening method for food safety risk monitoring.

Key words: Ultra-high performance liquidchromatography-tandem mass spectrometer, Solid phase extraction, Quinolones, Tetracycline, Chloramphenicol, Chicken gizzard, Chicken liver