Effects of occupational exposure to styrene on proliferation,invasion,apoptosis and cell cycle of leukemia cell line HL-60

Abstract

Abstract: Objective To explore the effect of occupational exposure to styrene on the proliferation,invasion,apoptosis and cell cycle of leukemia cell line HL-60 based on the miR-126-CSE1L pathway. Methods The leukemia cell line HL-60 in the logarithmic growth phase was exposed to styrene solutions of 0,10,20,and 40 nmoL/mL for 72 h. After culture,the cell viability and cell colony levels were measured using CCK-8 kit and crystal violet staining. Flow cytometry was used to measure cell cycle and apoptosis levels,Transwell assay was used to measure cell invasion levels,and RT-PCR and western blot methods were used to measure cellular miR-126 and CSE1L levels. Results After the leukemia cell line HL-60 was treated with styrene at doses of 10,20,and 40 nmoL/mL,the survival rate,cell colony,invasion,and CSE1L mRNA protein expression levels（0.68±0.18 vs 1.18±0.17,1.51±0.15,2.75±0.11;0.26±0.12 vs 0.50±0.11,0.79±0.14,1.24±0.18） were significantly higher than those in the control group,G2/M phase,apoptosis rate,and miR-126 expression level（2.97±0.20 vs 2.24±0.19,1.65±0.14,0.85±0.13） were significantly lower than those in the control group,and the differences were statistically significant（all P<0.05）. The survival rate,cell colony,invasion,and CSE1L mRNA protein expression levels（1.18±0.17,1.51±0.15,2.75±0.11,0.50±0.11,0.79±0.14,1.24±0.18） gradually increased in the styrene exposure dose groups of 10,20,and 40 nmoL/mL,while the G2/M phase,apoptosis rate,and miR-126 expression levels（2.24±0.19,1.65±0.14,0.85±0.13） gradually decreased. Styrene increased the survival rate,cell colonies,G2/M phase,invasion,and CSE1L mRNA protein expression levels in each group in a dose-dependent manner,while reduced G2/M phase,apoptosis rate,and miR-126 expression levels in each group,and the differences were statistically significant（all P<0.05）. Conclusion Styrene can promote the proliferation and invasion of leukemia cell line HL-60,inhibit apoptosis,and induce cell cycle arrest. The mechanism may be related to the inhibition of miR-126 levels in leukemia cell line HL-60 by styrene,which in turn targets the up-regulation of CSE1L expression.

Key words: Styrene, miR-126, CSE1L, Leukemia, Proliferation, Invasion, Apoptosis, Cell cycle

YANG Jin. Effects of occupational exposure to styrene on proliferation,invasion,apoptosis and cell cycle of leukemia cell line HL-60. [J]OCCUPATION AND HEALTH, 2025, 41(11): 1483-1488.

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