Research progress on genotoxicity and mechanism of carbon black particles

Abstract

Abstract: Carbon black（CB） is a stable nano-scale industrial material,which has been widely used in rubber,tires,plastics,batteries,printing and other industries. Due to the small size of CB particles,they can enter the cell and cause damage to genetic material through direct or indirect effects. At present,the International Agency for Research on Cancer classifies CB as a suspected human carcinogen. Most in vitro and in vivo studies have confirmed the genotoxicity of CB. In animal studies,only rats have been tested positive for the carcinogenicity of CB,while the population epidemiological carcinogenic evidence and the studies of genetic damage mechanism of CB are relatively limited. Based on the toxicological data of CB,this paper systematically reviews the characteristics,toxicokinetics,occupational exposure,genotoxicity assessment,genotoxic mechanism and carcinogenic effects of CB,so as to provide scientific basis for the health risk assessment and the formulation of prevention and control measures of CB particles.

Key words: Carbon black particles, Genotoxicity, Oxidative stress, Inflammatory response, Cell cycle, Epigenetic inheritance

CHENG Xuan, YAO Yan, WANG Kun, ZHANG Siyu, ZHANG Zhidong, MENG Tao. Research progress on genotoxicity and mechanism of carbon black particles. [J]OCCUPATION AND HEALTH, 2025, 41(11): 1563-1568.

References

[1] OBERDORSTER G,SHARP Z,ATUDOREI V,et al.Extrapulmonary translocation of ultrafine carbon particles following kwhole-body inhalation exposure of rats[J].J Toxicol Environ Health A,2002,65(20):1531-1543.
[2] ZHANG R,DAI Y,ZHANG X,et al.Reduced pulmonary function and increased pro-inflammatory cytokines in nanoscale carbon black-exposed workers[J].Part Fibre Toxicol,2014,11:73.
[3] DUAN H,JIA X,ZHAI Q,et al.Long-term exposure to diesel engine exhaust induces primary DNA damage:A population-based study[J].Occup Environ Med,2016,73(2):83-90.
[4] BOURDON J A,SABER A T,JACOBSEN N R,et al.Carbon black nanoparticle instillation induces sustained inflammation and genotoxicity in mouse lung and liver[J].Part Fibre Toxicol,2012,9:5.
[5] HUSAIN M,KYJOVSKA Z O,BOURDON-LACOMBE J,et al.Carbon black nanoparticles induce biphasic gene expression changes associated with inflammatory responses in the lungs of C57BL/6 mice following a single intratracheal instillation[J].Toxicol Appl Pharmacol,2015,289(3):573-588.
[6] KYJOVSKA Z O,JACOBSEN N R,SABER A T,et al.DNA damage following pulmonary exposure by instillation to low doses of carbon black(Printex 90) nanoparticles in mice[J].Environ Mol Mutagen,2015, 56(1):41-49.
[7] 张宁. PLK1介导的信号在纳米级炭黑致遗传损伤中的作用研究[D].石家庄:河北医科大学,2019.
[8] DI IANNI E,MØLLER P,CHOLAKOVA T,et al.Assessment of primary and inflammation-driven genotoxicity of carbon black nanoparticles in vitro and in vivo[J].Nanotoxicology,2022,16(4):526-546.
[9] ZHANG R,ZHANG X,JIA C,et al.Carbon black induced DNA damage and conformational changes to mouse hepatocytes and DNA molecule:A combined study using comet assay and multi-spectra methods[J].Ecotoxicol Environ Saf,2019,170:732-738.
[10] PEI Z,NING J,ZHANG N,et al.Genetic instability of lung induced by carbon black nanoparticles is related with Plk1 signals changes[J].NanoImpact,2022,26:100400.
[11] 周丽晓. 纳米级炭黑颗粒对16HBE细胞毒性作用及影响因素的研究[D].石家庄:河北医科大学,2017.
[12] CHENG W,LIU Y,TANG J,et al.Carbon content in airway macrophages and genomic instability in Chinese carbon black packers[J].Arch Toxicol,2020,94(3):761-771.
[13] 程文婷. 职业人群长期暴露炭黑导致的外周血淋巴细胞基因组不稳定性研究[D].青岛:青岛大学,2020.
[14] TOTSUKA Y,HIGUCHI T,IMAI T,et al.Genotoxicity of nano/microparticles in in vitro micronuclei,in vivo comet and mutation assay systems[J].Part Fibre Toxicol,2009,6:23.
[15] DI GIORGIO M L,DI BUCCHIANICO S,RAGNELLI A M,et al.Effects of single and multi walled carbon nanotubes on macrophages:Cyto and genotoxicity and electron microscopy[J].Mutat Res,2011, 722(1):20-31.
[16] POMA A,LIMONGI T,PISANI C,et al.Genotoxicity induced by fine urban air particulate matter in the macrophages cell line RAW 264.7[J].Toxicol In Vitro,2006,20(6):1023-1029.
[17] SONIA,KOMAL,KUKRETI S,et al.Exploring the DNA damaging potential of chitosan and citrate-reduced gold nanoparticles:Physicochemical approach[J].Int J Biol Macromol,2018,115:801-810.
[18] CHAUDHURI I,FRUIJTIER-PÖLLOTH C,NGIEWIH Y,et al.Evaluating the evidence on genotoxicity and reproductive toxicity of carbon black:A critical review[J].Crit Rev Toxicol,2018,48(2):143-169.
[19] CORREIA B,LOURENÇO J,MARQUES S,et al.Oxidative stress and genotoxicity of an organic and an inorganic nanomaterial to eisenia andrei:SDS/DDAB nano-vesicles and titanium silicon oxide[J].Ecotoxicol Environ Saf,2017,140:198-205.
[20] MODRZYNSKA J,BERTHING T,RAVN-HAREN G,et al.Primary genotoxicity in the liver following pulmonary exposure to carbon black nanoparticles in mice[J].Part Fibre Toxicol.2018,15(1):2.
[21] DI IANNI E,JACOBSEN N R,VOGEL U B,et al.Systematic review on primary and secondary genotoxicity of carbon black nanoparticles in mammalian cells and animals[J].Mutat Res Rev Mutat Res,2022, 790:108441.
[22] 张夏男. 石英和炭黑对MAPK/AP-1信号通路的影响[D].北京:中国疾病预防控制中心,2015.
[23] 董一文,张夏男,刘凯,等.ERK/JNK信号通路在炭黑诱导细胞因子IL-6、IL-8表达改变中的作用[J].卫生研究,2021,50(1):46-50.
[24] HIRAKU Y,NISHIKAWA Y,MA N,et al.Nitrative DNA damage induced by carbon-black nanoparticles in macrophages and lung epithelial cells[J].Mutat Res Genet Toxicol Environ Mutagen,2017,818:7-16.
[25] 孟涛,杨墨,高晔,等.吸入炭黑气溶胶致小鼠肺和外周血的炎性改变及相关性分析[J].第三军医大学学报,2018,40(18):1636-1643.
[26] TANG J,CHENG W,GAO J,et al.Occupational exposure to carbon black nanoparticles increases inflammatory vascular disease risk:An implication of an ex vivo biosensor assay[J].Part Fibre Toxicol,2020, 17(1):47.
[27] 杨聚岭. miR-96靶向FOXO3a在纳米级炭黑致16HBE细胞毒性中的作用研究[D].石家庄:河北医科大学,2018.
[28] 林慧. 组蛋白去乙酰化酶6在炭黑颗粒致小鼠肺部炎症中的作用[D].武汉:武汉科技大学,2020.
[29] DONALDSON K,TRAN L,JIMENEZ L A,et al.Combustion-derived nanoparticles:A review of their toxicology following inhalation exposure[J].Part Fibre Toxicol,2005,2:10.