Abstract: Objective To establish a high-performance liquid chromatography tandem time-of-flight mass spectrometry method and a high-resolution mass spectrometry database for the determination of 18 prohibited colorants in hair dye cosmetics,and explore the application in the detection of hair dye cosmetics. Methods Extracted with water：methanol：tetrahydrofuran at a ratio of 2 ∶ 2 ∶ 1,samples were separated using a Waters ACQUITY UPLC BEH C18 column. Gradient elution was performed using 2 mmol/L ammonium formate aqueous solution（containing 0.05% formic acid） and acetonitrile as the mobile phase. In the positive and negative ion mode of electric spray,the information dependent acquisition（IDA） mode was used for analysis,recording the retention times,accurate mass of primary and secondary ions,and abundance ratios of each component. Results A high-resolution quality database of 18 prohibited colorants in hair dye cosmetics was established and validated. This database was used to test 50 batches of hair dye products,and no traces of using these 18 prohibited colorants were found. Conclusion This method is easy to operate and highly sensitive,which can be used for qualitative screening of 18 prohibited colorants in hair dye cosmetics,providing a strong technical support for the safety supervision of hair dye cosmetics.

Key words: Cosmetics, Dye hair, Prohibit colorants, High resolution mass spectrometry, Mass spectrum database