Application and prospect of plant DNA barcoding in food

Abstract

Abstract: Several incidents on food adulteration were reported in recent times. Currently,China has not established appropriate standards to identify various food ingredients. However,in order to control food adulteration,various technologies such as spectrophotometer,gas chromatography-mass spectrometry（GC-MS）,high performance liquid chromatography（HPLC）,and DNA barcoding have been developed. Among these techniques,DNA barcoding is a fast,accurate,efficient and non-targeted biotechnology advantage with the principle of using 400-800 bp long standardized unique DNA sequences to analyze and classify the food commodities. This article reviews the common plant DNA barcoding and their applications in identifying legumes,oils,medicinal and edible homologous,spices,berry beverages,health foods,etc.,laying the foundation for the wider application of this technology in setting standards for quality assurance,quality control and food safety for different food products.

Key words: Plant, DNA barcoding, Food

CLC Number:

REN Ruijuan, LIU Xiang, YUE Mingxiang, WANG Hongjiao, LI Yongfa. Application and prospect of plant DNA barcoding in food[J]. OCCUPATION AND HEALTH, 2025, 41(17): 2429-2433.

References

[1] EMILY J V,CHRISTINE M.DNA barcoding for identification of consumer-relevant fungi sold in new york:A powerful tool for citizen scientists?[J].Foods,2018,7(6):87.
[2] QASIM M,WANG B,ZOU H,et al.Phylogenetic relationship and genetic diversity of citrus psyllid populations from China and Pakistan and their associated Candidatus bacterium[J].Mol Phylogenet Evol,2018,238(5):783.
[3] BLAXTER M,MANN J,CHAPMAN T,et al.Defining operational taxonomic units using DNA barcode data[J].Philos Trans R Soc Lond B Biol Sci,2005,1462(360):1935-1943.
[4] RACH J,DESALLE R,SARKAR I N,et al.Character-based DNA barcoding allows discrimination of genera,species and populations in Odonata[J].P Roy Soc B-Biol Sci,2008,275:237-247.
[5] FAZEKAS A J,BURGESS K S,KESANAKURTI P R,et al.Multiple multilocus DNA barcodes from the plastid genome discriminate plant species equally well[J].PLoS One,2008,3(7):e2802.
[6] CHEN S,HUI Y,HAN J,et al.Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species[J].PloS One,2010, 5(1):e8613.
[7] BAFEEL S O,ARIF I A,HOMAIDAN A A A,et al.DNA barcoding of arid wild plants using rbcL gene sequences[J].Genet Mol Res,2012, 11(3):1934-1941.
[8] LAHAYE R,BANK M,BOGARIN D,et al.DNA barcoding the floras of biodiversity hotspots[J].Proc Natl Acad Sci USA,2008,105(8):2923-2928.
[9] HEBERT P D N,CYWINSKA A,BALL S L,et al.Biological identifications through DNA barcodes[J].Proc Biol Sci,2003,270(1512):313-321.
[10] SENAPATI A,BASAK S,RANGAN L.A Review on application of DNA barcoding technology for rapid molecular diagnostics of adulterants in herbal medicine[J].Drug Saf,2022,45(3):193-213.
[11] MOON B C,KIM W J,HAN K S,et al.Differentiating authentic adeno-phorae radix from its adulterants in commercially-processed samples using multiplexed ITS sequence-based SCAR markers Medicine[J].Appl Sci,2017,7(7):660.
[12] CHASE M W,SOLTIS D E,OLMSTEAD R G,et al.Phylogenetics of seed plants:An analysis of nucleotide sequences from the plastid gene rbcL[J].Ann Missouri Bot Gard,1993,80(3):528-580.
[13] NURHASANA H,SUNDAR I,NURMAYA P,et al.Amplification and analysis of rbcL gene(ribulose-1,5-bisphosphate carboxylase) of clove in Ternate Island[J].IOP Conf Ser Earth Environ Sci,2019,276(1):12061.
[14] KANG Y,DENG Z,ZANG R,et al.DNA barcoding analysis and phylogenetic relationships of tree species in tropical cloud forests[J].Sci Rep,2017,7(1):12564.
[15] ZOSCHKE R,NAKAMURA M,LIERE K,et al.An organellar maturase associates with multiple group Ⅱ introns[J].Proc Natl Acad Sci USA,2010,107(7):3245-3250.
[16] SELVARAJ D,SARMA R K,SATHISHKUMAR R.Phylogenetic analysis of chloroplast matK gene from Zingiberaceae for plant DNA barcoding[J].Bioinformatio,2008,3(1):24-27.
[17] HILU K W,LIANG H P.The matK gene:Sequence variation and application in plant systematics[J].Am J Bot,1997,84(6):830-839.
[18] LOERA-SÁNCHEZ M,STUDER B,KÖLLIKER R.DNA barcode trnH-psbA is a promising candidate for efficient identification of forage legumes and grasses[J].BMC Res Notes,2020,13(1):35.
[19] SHAW J,LICKEY E B,SCHILLING E E,et al.Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms:The tortoise and the hare Ⅲ[J].Am J Bot, 2007,94(3):275-288.
[20] KRESS W J,ERICKSON D L.A two-locus global DNA barcode for land plants:The coding rbcL gene complements the non-coding trnH-psbA spacer region[J].PLoS One,2007,2(6):e508.
[21] VAN DE WIEL C C M,VAN DER SCHOOT J,VAN VALKENBURG J L C H.DNA barcoding discriminates the noxious invasive plant species, floating pennywort(Hydrocotyle ranunculoides L.f.),from non-invasive relatives[J].Mol Ecol Resour,2009,9(4):1086-1091.
[22] YAO H,SONG J Y,MA X Y,et al.Identification of dendrobium species by a candidate DNA barcode sequence:The chloroplast psbA-trnH intergenic region[J].Planta Med,2009,75(6):667-669.
[23] MA X Y,XIE C X,LIU C,et al.Species identification of medicinal pteridophytes by a DNA barcode marker,the chloroplast psbA-trnH intergenic region[J].Biol Pharm Bull,2010,33(11):1919-1924.
[24] WHITLOCK B A,HALE A M,GROFF P A.Intraspecific inversions pose a challenge for the trnH-psbA plant DNA barcode[J].PLoS One,2010,5(7):e11533.
[25] AlVAREZ I,WENDEL J F.Ribosomal ITS sequences and plant phylogenetic inference[J].Mol Phylogenet Evol,2003,29(3):417-434.
[26] CHINA PLANT BOL GROUP,LI D Z,GAO L M,et al.Comparative analysis of a large dataset indicates that internal transcribed spacer(ITS) should be incorporated into the core barcode for seed plants[J].Proc Natl Acad Sci U S A,2011,108(49):19641-19646.
[27] 程希婷,王爱民,顾志峰,等.DNA条形码研究进展[J].基因组学与应用生学,2011,30(6):748-758.
[28] 庞晓慧. 植物DNA条形码序列筛选与鉴定研究[D].北京:中国协和医科大学,2010.
[29] REED G H,WITTWER C T.Sensitivity and specificity of single-nucleotide polymorphism scanning by highresolution melting analysis[J].Clin Chem,2004,50(10):1748-1754.
[30] AMANE D,ANANTHANARAYAN L.Detection of adulteration in black gram-based food products using DNA barcoding[J].Food Control,2019, 104:193-200.
[31] MADESIS P,GANOPOULOS I,ANAGNOSTIS A,et al.The application of Bar-HRM(Barcode DNA-High Resolution Melting) analysis for authenticity testing and quantitative detection of bean crops(Leguminosae) without prior DNA purification[J].Food Control,2012,25(2):576-582.
[32] BOSMALI I,GANOPOULOS I,MADESIS P,et al.Microsatellite and DNA-barcode regions typing combined with high resolution melting(HRM) analysis for food forensic uses:A case study on lentils(Lens culinaris)[J].Food Res Int,2012,46(1):141-147.
[33] GANOPOULOS I,MADESIS P,DARZENTAS N,et al.Barcode high resolution melting(Bar-HRM) analysis for detection and quantification of PDO“Fava Santorinis”(Lathyrus clymenum) adulterants[J].Food Chem,2012,133(2):505-512.
[34] KUMAR S,KAHLON T,CHAUDHARY S.A rapid screening for adulterants in olive oil using DNA barcodes[J].Food Chem,2011,127(3):1335-1341.
[35] UNCU A T,UNCU A O,FRARY A,et al.Barcode DNA length polymorphisms vs fatty acid profiling for adulteration detection in olive oil[J].Food Chem,2017,221:1026-1033.
[36] PEREIRA L,GOMES S,BARRIAS S,et al.Applying highresolution melting(HRM) technology to olive oil and wine authenticity[J].Food Res Int,2018,103:170-181.
[37] ZUO Y,CHEN Z,KONDO K,et al.DNA barcoding of panax species[J].Planta Med,2011,77(2):182-187.
[38] NGUYEN V B,LINH G V N,WAMINAL N E,et al.Comprehensive comparative analysis of chloroplast genomes from seven Panax species and development of an authentication system based on speciesunique single nucleotide polymorphism markers[J].J Ginseng Res,2020,44(1):135-144.
[39] DHANYA K,SYAMKUMAR S P,SASIKUMAR B.Development and application of SCAR marker for the detection of papaya seed adulteration in traded black pepper powder[J].Food Biotechnol,2009,23(2):97-106.
[40] PARVATHY V A,SWETHA V P,SHEEJA T E,et al.DNA barcoding to detect chilli adulteration in traded black pepper powder[J].Food Biotechnol,2014,28(1):25-40.
[41] VILLA C,COSTA J,OLIVEIRA M B P P,et al.Novel quantitative real-time PCR approach to determine safflower(Carthamus tinctorius) adulteration in saffron(Crocus sativus)[J].Food Chem,2017,229(AUG.15):680.
[42] MATTIA F D,BRUNI I,GALIMBERTI A,et al.A comparative study of different DNA barcoding markers for the identification of some members of Lamiaceae[J].Food Res Int,2011,44(3):693-702.
[43] 李梅阁. 基于DNA条形码的浆果及其制品真伪鉴别技术研究[D].南京:南京农业大学,2016.
[44] 邱希斌. 基于ITS基因条码的保健食品中植物源性成分的鉴定[J].福建农业学报,2023,38(8):989-1003.
[45] HOLLINGSWORTH P M,FORREST L L,SPOUGE J L,et al.A DNA barcode for land plants[J].Proc Natl Acad Sci USA,2009,106(31):12794-12797.