Analysis of isolation,identification and MLST typing of Brucella in Tianjin from 2020 to 2022

Abstract

Abstract: Objective To understand the molecular epidemiological characteristics of brucellosis in Tianjin and provide theoretical basis for the prevention and control of brucellosis in Tianjin in the future. Methods Brucella samples from brucellosis patients in national brucellosis surveillance sentinel medical institutions in Tianjin from 2020 to 2022 were isolated and identified. BCSP31 polymerase chain reaction（BCSP31-PCR）,AMOS polymerase chain reaction（AMOS-PCR） and single factor serum agglutination test（A,M,R） were used to identify the species and biotype of the isolates. The isolates were sequenced and the data were analyzed by Multi-locus sequence typing（MLST）. The Brucella public data and the data of this experimental study were included in the analysis,and the phylogenetic tree was constructed with the Neighbor-joining clustering analysis using Mega XI software.Results Twelve brucellosis isolates were isolated from brucellosis patient samples collected from national brucellosis surveillance sentinel medical institutions in Tianjin from 2020 to 2022,of which one strain was brucellamelitensis biotype1,and the other 11 strains were brucellamelitensis biotype 3. The 12 strains of Brucella were all ST8,sharing ST8 with Shanghai,Fujian,Hebei,Shanxi and other provinces and cities,and were closely genetically related to ST39 from Guizhou Province,ST71 from Henan and Sichuan Province,ST101 from Sichuan Province and ST118 from Sichuan Province. Conclusion The main epidemic strain of Brucella in Tianjin is brucellamelitensis biotype 3,and the main genotype is ST8,which is consistent with the domestic dominant strain and the epidemic ST type. This is the first molecular typing study of Brucella isolated and identified from human sources in Tianjin,filling in the local data of Tianjin for the global MLST database,and better mastering the molecular epidemiology of Brucella in Tianjin.

Key words: Brucella, AMOS-polymerase chain reaction, Multi-locus sequence typing, Brucellamelitensis biotype 3, ST8

CLC Number:

R117

ZHENG Baolu, LI Wen, ZHANG Wei, LYU Likun, SU Cheng, LI Xiaoyan. Analysis of isolation,identification and MLST typing of Brucella in Tianjin from 2020 to 2022. [J]OCCUPATION AND HEALTH, 2025, 41(18): 2469-2473.

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