Study on the contamination status and virulence gene distribution of Vibrio parahaemolyticus in seafood sold in Tianjin City

Abstract

Abstract: Objective To investigate the contamination status and virulence gene distribution of Vibrio parahaemolyticus in seafood sold in Tianjin,and to analyze virulence gene and the genetic evolutionary relationships between strains using whole-genome sequencing(WGS). Methods From September to October 2024,a total of 30 seafood samples were collected,and V. parahaemolyticus was isolated and identified by conventional bacteriological methods. Polymerase chain reaction(PCR) was employed to detect the virulence genes(tlh,tdh,and trh). Whole-genome sequencing was performed on the isolated strains to characterize the virulence gene profiles,and bioinformatics tools were used to assess their genetic evolutionary relationships. Results V. parahaemolyticus nucleic acid was detected in 36.67%(11/30) of the 30 enriched samples,with tlh gene positivity in 33.33%(10/30) and tdh and trh genes detected in 6.67%(2/30). Six strains of V. parahaemolyticus were isolated,all of which tested positive for tlh(20.00%,6/30),whereas tdh and trh genes were completely absent. WGS analysis revealed that the virulence genes were primarily associated with adhesion and effector delivery system. Comparative genomic analysis showed that the type Ⅲ secretion system 1(T3SS1) gene in the isolated strains shared 94%-100% homology with the reference strain RIMD2210633,indicating certain sequence variations. Conclusion The prevalence of V. parahaemolyticus in seafood sold in Tianjin is relatively high. WGS analysis indicates that virulence-associated genes are mainly involved in adhesion and effector delivery system,suggesting their crucial role in pathogenesis. The high homology of the T3SS1 gene suggests its evolutionary conservation among strains. This study provides molecular evidence for epidemiological surveillance and food safety risk assessment.

Key words: Seafood, Vibrio parahaemolyticus, Virulence genes, T3SS1

CLC Number:

R155.5

NING Peiyong, FAN Xiaoqing, ZOU Ming, LUO Sha, ZHUO Yue, LI Xiaoyan. Study on the contamination status and virulence gene distribution of Vibrio parahaemolyticus in seafood sold in Tianjin City. [J]OCCUPATION AND HEALTH, 2025, 41(21): 2918-2922.

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