Experimental study on the effect of temperature and PM2.5 exposure on alveolar macrophages in mice

Abstract

Abstract: Objective To investigate the toxic effects and mechanisms of different temperatures and fine particulate matter（PM_2.5） exposure on alveolar macrophages（AMs）. Methods The AMs of mice were extracted and cultured at 27,32,37 and 42 ℃,respectively,each of which was treated with different concentrations of PM_2.5. After 12 hours of exposure,the levels of AMs relative indicators in mice were detected. Results At the same exposure dose,the relative survival rate and phagocytic function of cells in the normal temperature group were the highest,and the phagocytic function of cells decreased with increasing or decreasing temperature. At the same temperature,the phagocytic ability of cells in the PM_2.5 treatment group was significantly lower than that in the control group,and the higher the PM_2.5 concentration,the lower the relative survival rate of cells and the stronger the toxic effect. A low temperature combined higher concentrations of PM_2.5 exposure could cause the levels of interleukin-6（IL-6） and C-reactive protein（CRP） increased significantly compared with the control group and the normal temperature group（P<0.05）. However, compared with the normal temperature group,there were no significant increase in the levels of macrophage inflammatory protein 1α（MIP-1α） in each group（all P>0.05）. With the increase of PM_2.5 exposure dose,the activity of superoxide dismutase（SOD） in mouse AMs significantly decreased and the level of malondialdehyde（MDA） significantly increased（both P<0.05）. In addition,the larger the temperature difference,the higher the PM_2.5 concentration,and the more obvious the effect on SOD and MDA in mouse AMs. Conclusion Different temperatures and exposure to PM_2.5 can have toxic effects on mouse AMs cells,which may be mediated by inducing cellular inflammatory responses and oxidative stress damage,leading to a decrease in phagocytic function. Additionally,there is an interactive effect between low temperature and high-dose PM_2.5 exposure on AMs.

Key words: Temperature, Fine particulate matter, Alveolar macrophages, Interaction

CLC Number:

R114

SONG Quanquan, LIU Shuqin, SUN Wu, WANG Xiuzhen, LI qian, ZHANG Shuyu. Experimental study on the effect of temperature and PM_2.5 exposure on alveolar macrophages in mice. [J]OCCUPATION AND HEALTH, 2025, 41(7): 899-903.

References

[1] LIU K,ZHANG H,BO Y C,et al.Ambient air pollution and Children's health:An umbrella review[J].Atmos Pollut Res,2024,15(6):102108.
[2] WU Y,SONG P,LIN S A,et al.Global burden of respiratory diseases attributable to ambient particulate matter pollution:Findings from the global burden of disease study 2019[J].Front Public Health,2021,9:74080.
[3] LI H P,LIANG L Z,ZHANG S Y,et al.Short-term ambient particulate matter pollution of different sizes and respiratory hospital admission in the Beibu Gulf area of Southern China[J].Atmos Environ,2023,294:119524.
[4] WANG H Y,SHEN X Y,LIU J,et al.The effect of exposure time and concentration of airborne PM_2.5 on lung injury in mice:A transcriptome analysis[J].Redox Biol,2019,26:101264.
[5] MORROW P E.Possible mechanisms to explain dust overloading of the lungs.Fundamental and applied toxicology:Official[J].J Soci Toxic,1988, 10:369-384.
[6] 付桂琴,田亚芹,张成伟.气温与臭氧协同作用对COPD住院人数的影响[J].中国环境科学,2019,39(12):5312-5318.
[7] 张书余,张夏琨,周骥,等.冷空气与霾对动脉粥样硬化大鼠影响的实验研究[J].科学技术与工程,2018,18(2):56-65.
[8] TCHICAYA A,LORENTZ N,OMRANI H,et al.Impact of long-term exposure to PM_2.5 and temperature on coronavirus disease mortality:Observed trends in France[J].J Environ Health,2021,20(1):101.
[9] 高衍新,孙文,杜英林,等.气温与PM_2.5交互作用对呼吸系统疾病就诊风险的影响研究:基于山东省四城市分析[J].环境卫生学杂志,2023,13(1):30-36.
[10] SUJUAN C,HANG D,MENGMENG L,et al.Interactive effects between temperature and PM_2.5 on mortality:A study of varying coefficient distributed Lag Model-Guangzhou,Guangdong Province,China,2013-2020[J].China CDC weekly,2022,4(26):570-576.
[11] 罗斌,晚亚雄,石艳荣,等.低温刺激与PM_2.5对大鼠肺组织及系统炎症因子的影响[J].科学技术与工程,2015,15(7):30-33.
[12] 张凯,费高强,李兴杰,等.高温及低温大气PM_2.5对大鼠肺泡巨噬细胞的毒性作用[J].环境与健康杂志,2017,34(5):394-397.
[13] 罗斌,刘江涛,费高强,等.不同温度刺激与大气PM_2.5对大鼠肺泡巨噬细胞的交互毒性作用研究[J].中国应用生理学杂志,2017,33(1):71-75.
[14] 王甜,嵇志刚,刘蓉,等.大气PM_2.5诱导小鼠单核巨噬细胞自噬及其作用机制[J].环境与健康杂志,2024,41(2):111-114,189.
[15] 王宏,罗深秋,王应斌,等.高温对整体大鼠腹腔巨噬细胞吞噬功能及凋亡的影响[J].中华劳动卫生职业病杂志,2001,19(3):42-44.
[16] MOURTZOUKOU E G,FALAGAS M E.Exposure to cold and respiratory tract infections[J].Int J Tuberc Lung Dis,2007,11(9):938-943.
[17] EEDEN S F,YEUNG A,QUINLAM K,et al.Systemic response to ambient particulate matter:Relevance to chronic obstructive pulmonary disease[J].Proc Am Thorac Soc,2005,2:61-67.
[18] 王广鹤,甄玲燕,吕鹏.臭氧和细颗粒物暴露对大鼠心脏自主神经系统和系统炎症的影响[J].卫生研究,2013,42(4):554-560.
[19] WANG C L,ZHANG S Y,et al.TIAN Y.Effects of simulated heat waves on ApoE-/- mice[J].Int J Env Res Pub He,2014,11(2):1549-1556.
[20] LUO B,SHI H,WANG L,et al.Rat lung response to PM_2.5 exposure under different cold stresses[J].Int J Env Res Pub He,2014,11:12915-12926.
[21] 易建华,吴晓芳,王丽云,等.PM_2.5对呼吸系统疾病的影响及其机制的研究进展[J].西安交通大学学报(医学版),2019,40(1):167-172.
[22] SU R,JIN X,ZHANG W,et al.Particulate matter exposure induces the autophagy of macrophages via oxidative stress-mediated PI3K/AKT/mTOR pathway[J].Chemosphere,2017,167:444-453.

Experimental study on the effect of temperature and PM_2.5 exposure on alveolar macrophages in mice

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