Effects of occupational cadmium exposure on malignant biological behavior of leukemia cell line HL-60 and expression of circRNA-SMAD7 and Tspan15

Abstract

Abstract: Objective To explore the effects of occupational cadmium exposure on the malignant biological behavior of leukemia cell line HL-60 and the expression of circular RNA-SMAD7（circ-SMAD7） and tetraspanin15（Tspan15）. Methods The leukemia cell line HL-60 in the logarithmic growth phase was exposed to 0,250,500,and 1 000 nmol/L cadmium chloride solutions for 72 hours. MTT kit and crystal violet staining were used to measure cell viability and cell colony level. The flow cytometry was used to measure cell cycle and apoptosis level. Transwell and scratch experiments were used to measure cell invasion and migration level. RT-PCR and Western blot methods were used to measure cellular circRNA-SMAD7 and Tspan15 level. Results Compared with the leukemia cell line HL-60 group,the survival rate,number of monoclonal formation,number of membrane penetrations,and migration distance of the low-,medium-,and high-dose cadmium chloride groups were increased,and the apoptosis rate,cell cycle G2/M phase,and the level of circRNA-SMAD7（6.39±0.63 vs 4.88±0.43,3.23±0.35,1.89±0.17） decreased（P<0.05）,while the level of Tspan15 mRNA protein（1.15±0.10 vs 2.31±0.21,3.25±0.33,4.80±0.41,0.26±0.05 vs 0.52±0.10,0.82±0.15,1.59±0.23） increased（P<0.05）. With the increase in the amount of cadmium chloride,the survival rate,the number of monoclonal formation,the number of membrane penetration and migration distance gradually increased in cadmium chloride groups,and the apoptosis rate,cell cycle G2/M phase,and the level of circRNA-SMAD7 gradually decreased,while the level of Tspan15mRNA protein gradually increased,and the differences were all statistically significant（all P<0.05）. Conclusion The cadmium can promote the proliferation and invasion of leukemia cell line HL-60,inhibit apoptosis,induce cell cycle arrest,and its mechanism may be related to the fact that cadmium inhibits the level of leukemia cell line HL-60 circ-SMAD7,thereby targeting up-regulation of Tspan15 expression.

Key words: Cadmium, Leukemia, Malignant biological behavior, circRNA-SMAD7, Tspan15

CLC Number:

R114

YANG Jin. Effects of occupational cadmium exposure on malignant biological behavior of leukemia cell line HL-60 and expression of circRNA-SMAD7 and Tspan15. [J]OCCUPATION AND HEALTH, 2025, 41(7): 904-910.

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