Evaluation of uncertainty for validation of tartrazine capacity in fruit juice

Abstract

Abstract: Objective To evaluate the uncertainty of tartrazine measurement results of quality control samples and blind samples in capability verification using high performance liquid chromatography,identify the main influencing factors of uncertainty,and provide reference for improving the reliability and accuracy of detection results. Methods Referring to GB 5009.35-2016 "National Food Safety Standard-Determination of Synthetic Colorants in Food",the pre-treatment method was optimized,enrichment and purification by solid-phase extraction column（SPE）,the sample was determined by constant volume and filter. According to JJF 1059.1-2012 "Evaluation and Expression of Measurement Uncertainty",the sources of uncertainty of the determination of tartrazine content in fruit juice were analyzed,including instrument,standard substances and sample pretreatment. By establishing a mathematical model and calculating the uncertainty components,the synthetic standard uncertainty and extended uncertainty were given. Results The determination of quality control samples was within the allowable error range of the specified value. According to the confidence interval of 95%,the extended uncertainties for the measured of tartrazine content in samples G and E were 5.31 and 3.88 μg/kg（k=2）. Conclusion The sources of uncertainty in the determination of tartrazine content in fruit juice mainly come from the preparation process of standard solutions and the repeatability of liquid chromatography. PWAX SPE columns can be used for sample pr-treatment,and regular periodic checks and instrument maintenance can be carried out to reduce the level of uncertainty.

Key words: Tartrazine, High performance liquid chromatography, Capability validation, Uncertainty, Fruit juice

CLC Number:

R115

MA Xiaonian, YANG Cheng, CHEN Zeqi, CHEN Junxiu, NONG Ruiyu, LI Yansheng. Evaluation of uncertainty for validation of tartrazine capacity in fruit juice. [J]OCCUPATION AND HEALTH, 2025, 41(8): 1035-1040.

References

[1] 杨靖研,杨璐幪,李海防.蜜饯中5种着色剂使用现状的调查研究[J].现代食品,2022,28(8):225-228.
[2] HANNANEH R,MOHAMMAD S M,ENAYAT B,et al.I dentification of tartrazine adulteration and evaluating exposure to synthetic dyes of sunset yellow and quinoline yellow through consumption of food products among children[J].Food Sci Nutr,2022,10(11):3781-3788.
[3] NURSENA D,MEHMET G,NURCAN G,et al.Thymoquinone played a protective role against tartrazine-induced hepatotoxicity[J].Iran J Basic Med Sci,2023,26(1):99-106.
[4] CIGDEM V,E M E,MEHMET G,et al.Protective effect of crocin on food azo dye tartrazine-induced hepatic damage by improving biochemical parameters and oxidative stress biomarkers in rats[J].Gen Physiol Biophys,2019,38(1):73-82.
[5] STEPHANE Z,NDJENGUE L E M,CHARLINE F A,et al.Oral administration of tartrazine(E102) accelerates the incidence and the development of 7,12-dimethylbenz(a) anthracene(DMBA)-induced breast cancer in rats[J].BMC Complement Med,2021,21(1):303.
[6] WOPARA I,O A A,G O A,et al.Combine colorants of tartrazine and erythrosine induce kidney injury:involvement of TNF-α gene,caspase-9 and KIM-1 gene expression and kidney functions indices[J].Toxicol Mech Method,2021,31(1):67-72.
[7] 许彤,张毅,严恒,等.分散固相萃取-高效液相色谱法测定饮料和糖果中3种人工合成色素[J].食品安全质量检测学报,2022,13(14):4590-4597.
[8] 梁剑锋,李亚,柯立坚,等.液液萃取-高效液相色谱法测定奶茶中六种合成色素含量[J].中国食品添加剂,2022,33(9):190-195.
[9] 许彬,林金海,张少虹,等.固相萃取-高效液相色谱法测定蜜饯中7种合成着色剂的含量[J].中国食品添加剂,2021,32(3):70-75.
[10] XIAO W Z,YI X Y,PEI G Q,et al.Facile preparation of nano-g-C3N4/UiO-66-NH2 composite as sorbent for high-efficient extraction and preconcentration of food colorants prior to HPLC analysis[J].Chinese Chem Lett,2022,33(2):903-906.
[11] LI R,WANG Y,TAN J,et al.Simultaneous determination of synthetic edible pigments in beverages by titania-based RP-HPLC[J].Arab J Chem,2020,13(2):3875-3881.
[12] 潘颖,吕沈亮,夏苏捷,等.超高效液相色谱-串联质谱法测定鱼肉组织中9种黄色染色剂[J].中国食品卫生杂志,2022,34(1):44-48.
[13] 陈燕,吕沈亮,李永吉,等.超高效液相色谱-串联质谱法同时检测食品中30种食品添加剂[J].中国卫生检验杂志,2021,31(2):134-140.
[14] ALWARTHAN A A,SIDDIQUI R M,KHAN A M,et al.Corncob waste based adsorbent for solid phase extraction of tartrazine in carbonated drinks and analytical method using ultra performance liquid chromatography-mass spectrometry[J].Curr Anal Chem,2020,16(7):924-932.
[15] MA K,LI X,WANG H,et al.Rapid and sensitive method for the determination of eight Food additives in red wine by ultra-performance liquid chromatography tandem mass spectrometry[J].Food Anal Method,2015,8(1):203-212.
[16] 薄梦阳. TLC-SERS联用快速检测食品中人工合成色素的研究[D].天津:天津科技大学,2018.
[17] NAMBIAR P A,SANYAL M,SHRIVASTAV S P.Simultaneous densitometric determination of eight food colors and four sweeteners in candies,jellies,beverages and pharmaceuticals by normal-phase high performance thin-layer chromatography using a single elution protocol[J].J Chromatogra A,2018,1572:152-161.
[18] 郭光美,李景印,李淑芳.偏最小二乘-分光光度法同时测定食品中多组分人工合成色素[J].食品工业,2014,35(10):15-17.
[19] ZAHRA M,SHAHLA E,VAHID Z,et al.Fe3O4@PDA@PANI core-shell nanocomposites as a new adsorbent for simultaneous preconcentration of tartrazine and sunset yellow by ultrasonic-assisted dispersive micro solid-phase extraction[J].Spectrochim Acta A,2021,262:120130.
[20] BIŞGIN A T.Simultaneous spectrophotometric determination of brilliant blue and tartrazine in diverse sample matrices after solid phase extraction[J].J AOAC Int,2020,103(6):1478-1485.
[21] HYOSUB L,GUNHEE J,JUHYEON M,et al.Korean proficiency tests for pesticide residues in rice:Comparison of various proficiency testing evaluation methods and identification of critical factors for multiresidue analysis.[J].Foods(Basel,Switzerland),2023,12(10):2085.
[22] 康婕,李媛,石菲,等.饮料中乙酰磺胺酸钾(安赛蜜)的测定能力验证[J].食品安全导刊,2022(9):58-61,65.
[23] 张伟清,侯俐南,乔亚森,等.饮料中柠檬黄的测定能力验证情况分析[J].食品安全质量检测学报,2020,11(4):1255-1260.
[24] 董丽荣,刁玉华,安宁,等.基于质控样测试的内部质量控制结果分析[J].中国标化,2023(21):214-218.
[25] 宫赤霄,彭强.实验室质量监控中的新角色[J].质量与认证,2018(7):72-74.
[26] 张慧琼,郑陆红,陈嘉敏.高效液相色谱法测定蜜饯中柠檬黄的不确定度评定[J].现代食品,2022,28(23):195-198,208.
[27] 蒋小波,张勇.饮料中合成色素柠檬黄含量测量结果的不确定度评定[J].中国计量,2021(12):106-108.
[28] 刘惠霞,邓树强.药用胶囊中6种合成色素高效液相色谱法的测定及不确定度[J].中国药物经济学,2020,15(2):41-46.
[29] 张莉丽. 高效液相色谱法测定葡萄酒中柠檬黄、苋菜红的不确定度分析[J].计量与测试技术,2017,44(3):110-111,113.
[30] 王玉辉,康菁,王涛,等.液相色谱法测定葡萄酒中柠檬黄、苋菜红、胭脂红、日落黄含量结果的不确定度评定[J].食品安全质量检测学报,2017,8(1):280-285.
[31] 国家质量监督检验检疫总局.常用玻璃量器:JJG 196—2006[S].北京:中国计量出版社,2006:1-22.
[32] 中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品中合成着色剂的测定:GB 5009.35—2016[S].北京:中国标准出版社,2016:323-330.