Abstract:

Objective To improve the rapid detection of foodborne pathogenic bacteria，the application of real-time fluorescence reverse transcription polymerase chain reaction（RT-PCR） in active surveillance of foodborne diseases was explored and its effect was evaluated. Methods Stool samples were collected from active monitoring diarrhea patients in sentinel hospitals in Beijing from January to December 2024. Salmonella，Vibrio parahaemolyticus and Campylobacter were detected by both traditional culture method and RT-PCR. The detection positive rates of three foodborne pathogenic bacteria were compared between RT-PCR and traditional culture method，and the application value of RT-PCR in foodborne pathogen detection was evaluated. Chi-square test，Kappa consistency test and receiver operating characteristic（ROC） curve were used to analyze the difference between the detection results. Results The positive rates of Salmonella，Vibrio parahaemolyticus and Campylobacter detected by RT-PCR method were 4.31%，4.35% and 13.83%，respectively. The positive rates of three foodborne pathogens detected by culture method were 5.13%，3.40% and 8.00%，respectively，and the differences in positive rates were statistically significant（χ²=984.879，1350.890，857.971，all P<0.01）. Compared with the culture method，the Kappa values of Salmonella，Vibrio parahaemolyticus and Campylobacter detected by RT-PCR method were 0.657，0.766 and 0.597，respectively. The area under the curve（AUC） of Salmonella，Vibrio parahaemolyticus and Campylobacter detected by RT-PCR method were 0.858，0.843 and 0.767，respectively. Conclusion The RT-PCR method has high specificity and authenticity in detecting Salmonella，Vibrio parahaemolyticus and Campylobacter，and has practical value in active monitoring of foodborne diseases. The RT-PCR method can be combined with culture methods to detect foodborne pathogens quickly and accurately，and provide technical support for the rapid response to foodborne disease events.

Key words: Salmonella, Vibrio parahaemolyticus, Campylobacter, Real-time fluorescence reverse transcription polymerase chain reaction, Foodborne diseases