Effects of lycium barbarum polysaccharide on behavioral ability and PI3K/AKT pathway activation in sleep deprivation rats

Abstract

Abstract: Objective To analyze the effects of lycium barbarum polysaccharide on autophagy protein,phosphatidylinositol 3 kinase（PI3K）/threonine protein kinase（AKT） signaling pathway and behavioral ability in hippocampus of sleep deprivation rats,and explore the molecular mechanism of lycium barbarum polysaccharide improving sleep deprivation rats. Methods The sleep deprived experimental was established in rats by using the "multiplatform water environment",18 rats were arranged into three groups：sleep deprivation group,lycium barbarum polysaccharide group and estazolam group. The health rats were arranged into the normal group. The lycium barbarum polysaccharide group and estazolam group were given daily gavage with lycium barbarum polysaccharide and estazolam respectively,sleep deprivation group and normal groupwere given daily gavage with the same volume of triple distilled water. All the four groups were continuously fed for 28 days. The changes of rats' behavioral abilities was measured through the "water maze" experiment. The enzyme-linked immunosorbent assay was utilized to measure the level of malondialdehyde（MDA） and superoxide dismutase（SOD） in hippocampus. The Western blot was utilized to determine the levels of bcl-2 homologous domain protein antibody（Beclin 1）,microtubule-associated protein light chain 3Ⅱ（LC3Ⅱ）/microtubule-associated protein light chain 3Ⅰ（LC3Ⅰ）,PI3K,phosphorylated inositol 3 kinase（p-PI3K）,Akt and phosphorylated serine threonine kinase（p-AKT） in the hippocampus of rats. Results Compared with the normal group,the rats’ escape latency[（41.98±5.27）s] in the sleep deprivation group was prolonged,and the number of crossing platforms[（9.56±1.46）times] was reduced（both P<0.05）,the content of MDA[（3.55±0.46）mmol/L] increased and the content of SOD[（7.62±1.20）U/L] decreased in the hippocampus tissue （both P<0.01）,the contents of autophagic proteins Beclin 1（1.60±0.17） and LC3Ⅱ/LC3Ⅰ（1.18±0.14） in the hippocampus increased（both P<0.05）,while the contents of p-PI3K/PI3K（0.28±0.05） and p-AKT/AKT（0.40±0.07） decreased（both P<0.05）. Compared with the sleep deprivation group,the rats’ escape latency[（29.79±4.01）s] in the ycium barbarum polysaccharide group was is shortened,and the number of crossing platforms[（16.01±2.29）times] was increased（both P<0.05）,the content of MDA[（2.31±0.38）mmol/L] decreased and the content of SOD[（14.25±1.89）U/L] increased in the hippocampus tissue（both P<0.01）,the contents of autophagic proteins Beclin 1（1.02±0.16） and LC3Ⅱ/LC3Ⅰ（0.80±0.12） in the hippocampus decreased（both P<0.05）,while the contents of p-PI3K/PI3K（0.52±0.08）and p-AKT/AKT（0.64±0.09）increased（both P<0.05）. The lycium barbarum polysaccharide group and estazolam group had not obvious difference in the above indicators（both P>0.05）. Conclusion The lycium barbarum polysaccharide is able to upgrade the behavioral ability of sleep deprivation rats by activating PI3K/Akt pathway and inhibiting excessive autophagy in hippocampus.

Key words: Lycium barbarum polysaccharides, Sleep deprivation, Autophagy protein, PI3K/AKT pathway, Behavioral ability

CLC Number:

R114

WANG Yanwei, SHAN Tieqiang, WANG Congmin, JIANG Liu, HAN Changhai, SHI Weigang. Effects of lycium barbarum polysaccharide on behavioral ability and PI3K/AKT pathway activation in sleep deprivation rats. [J]OCCUPATION AND HEALTH, 2024, 40(8): 1041-1045.

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