Abstract: Objective The immunofluorescence technology is the earliest developed detection technology with high sensitivity and strong specificity.It is expected to use this technology to prepare a low toxicity,economic and efficient carbon dots as a marker of immunofluorescence,label it on deoxynivalenol（DON） antibodies,so as to identify DON in samples by using this immunoprobe. Methods The polyvinylimide was used as surface modifier and citric acid as carbon source to prepare a carbon dots which could be labeled on antibody by using the microwave-assisted method. The amino/carboxyl coupling method was used to bind this carbon dot with DON antibodies to produce an immunofluorescence probe,and the binding of the probe with DON was observed under the microscope. Results When the excitation light was 334 nm,this carbon dots could emit a strong blue light of 445 nm. The infrared characterization showed that the surface of this carbon spot contains carboxyl group and amino groups modified by PEI,so it could be combined with the surface of the antibody containing amino group and could emit fluorescent signals. As an immunofluorescence probe to detect the corresponding antigen,fluorescence signals were observed under a fluorescence microscope. Conclusion The immunofluorescence probe of DON is prepared by combining the prepared fluorescent carbon dots with antibodies by amino/carboxyl coupling method,and this immune probe is successfully used for detecting DON.

Key words: Carbon dots, Antibody, Immunologic method, Immunofluorescence probe