Evaluation of uncertainty in the determination of perchlorate in the flower of Coffea by ultra-performance liquid chromatography-tandem mass spectrometry

Abstract

Abstract: Objective To determine the content of perchlorate in the flower of Coffea samples by ultra-performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）,and to evaluate the uncertainty of the testing process. Methods To analyze and calculate various uncertainty components in the detection process according to JJF 1059.1-2012 "the Evaluation and Expression of Uncertainty in Measurement",and finally synthesizing the extended uncertainty for the determination of perchlorate content in the flower of Coffea samples by UPLC-MS/MS. Results In this experiment,the content of perchlorate in the flower of Coffea samples was （0.131 3±0.012 2）mg/kg,k=2,and the relative standard deviation was 2.39%. Conclusion In the determination of perchlorate content in the flower of Coffea by UPLC-MS/MS,the uncertainty are mainly derived from the pipette absorbing isotope internal standard in the process of preparing standard series,the glass gauge and the pipette during the dilution process of standard materials,the determination and fitting of the standard curve.

Key words: Flower of Coffea, Perchlorate, Ultra-performance liquid chromatography-tandem mass spectrometry, Uncertainty

CLC Number:

R115

LI Chengxi, BAI Yinghao, QING Xueqin. Evaluation of uncertainty in the determination of perchlorate in the flower of Coffea by ultra-performance liquid chromatography-tandem mass spectrometry. [J]OCCUPATION AND HEALTH, 2024, 40(19): 2623-2629.

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