Effect of sodium fluoride on microstructure of bone cortex in experimental mice

Abstract

Abstract: Objective To investigate the effects of sodium fluoride（NaF） on the microstructure and strength of bone cortex in experimental mice. Methods 30 ICR male mice were randomly divided into three groups according to the principle of weight balance：the control group was given tap water regularly,the low fluoride group was given tap water containing 25 mg/L NaF,and the high fluoride group was given tap water containing 50 mg/L NaF. All groups were given free diet.After 20 weeks of feeding,the incidence of dental fluorosis in each group was observed. The left femur was scanned by the Micro CT,and 2D and 3D physical parameters were recorded. The bone fluoride was measured with fluoride ion electrode. The three-point bending test was performed on the left tibia. Results The mice in both low and high fluoride groups had dental fluorosis. Compared with the control group,the bone fluoride content[（1 023.37±175.42）μg/g] and the structural diameter in the low fluoride group and the bone fluoride content[（1 135.23±69.01）μg/g],section thickness,structural thickness,and structural diameter in the high fluoride group increased significantly,while the bone structural strength in the high fluoride group decreased significantly.Compared with the low fluoride group,the section thickness[（0.138±0.004）mm],structure thickness[（0.091±0.003）mm],structure diameter[（0.181±0.006）mm],bone cortex density[（1.978±0.009）g/cm³],bone volume fraction[（87.267±0.379）%] and bone structure strength[（3.74±1.00）kg] in the high fluoride group were significantly reduced. Conclusion The high dose sodium fluoride can destroy the microstructure of bone cortex in experimental mice and reduce the strength of bone structure.

Key words: Fluorosis, Bone cortex, Bone microstructure

CLC Number:

R-332

WU Li, LI Yangjie, YANG Fengmei, LIU Jie, SUN Lu, ZHANG Zhuo. Effect of sodium fluoride on microstructure of bone cortex in experimental mice. [J]OCCUPATION AND HEALTH, 2023, 39(16): 2189-2192.

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