Detection,virulence and serum analysis of Escherichia coli in drinking water in Liaoning Province

Abstract

Abstract: Objective To understand the situation of domestic drinking water polluted by Escherichia coli in some areas of Liaoning Province,and provide reference basis for the prevention and control of water-based diseases,as well as for the monitoring and early warning of the hygiene and safety of drinking water. Methods The microbial indicators of Escherichia coli in various kinds of drinking water in Liaoning Province from 2010 to 2021 were detected by multi tube fermentation method,and the pathogenic virulence genes and O antigen serotypes of the isolated Escherichia coli were identified and analyzed by polymerase Chain reaction（PCR） method. Results A total of 78 strains of Escherichia coli were detected,with a total sample detection rate of 11.84%. The treatment methods of water samples have significant differences in the detection of Escherichia coli indicators in water samples,and the qualification rate of non disinfected samples is significantly lower than that of disinfected water samples.Among them,16 strains of diarrhea causing Escherichia coli were detected,with a total detection rate of 0.024%. The detection rate of diarrhea causing Escherichia coli in source water was significantly higher than that in other types of samples.Four strains of Enteropathogenic Escherichia coli,five strains of Enterotoxigenic Escherichia coli and seven strains of Enteroaggregative Escherichia coli were detected from 16 strains of diarrhoeal Escherichia coli. Except five strains that could not be classified,the remaining 11 strains were classified into eight Serotype. Both virulence genes and O antigens have dominant typing. Conclusions The detection rate of Escherichia coli in various types of drinking water sources in Liaoning Province is the highest,and disinfection treatment of drinking water is crucial for reducing the detection rate of Escherichia coli.Escherichia coli causing diarrhea has been detected in source water,decentralized water supply,and centralized water supply. It has dominant virulence genes,and the detection rate of heat source stability genes is higher. Similarly,among the three kinds of Escherichia coli causing diarrhea,two of them have dominant O antigen.Therefore,the hygienic indicators of Escherichia coli in drinking water should be continuously monitored.

Key words: Drinking water, Escherichia coli, Diarrheagenic Escherichia coli, Virulence genes, O antigen

CLC Number:

R123

WEI Tongzhu, LI Xue, ZHANG Xu, YU Miao, WANG Weijie, SUN Tingting. Detection,virulence and serum analysis of Escherichia coli in drinking water in Liaoning Province. [J]OCCUPATION AND HEALTH, 2023, 39(16): 2193-2197.

References

[1] 舒丽萍,陈曌君,张文辉.2015—2019年杭州市农村生活饮用水微生物污染与肠道传染病发病率关联性分析[J].现代预防医学,2020, 47(22):4201-4205.
[2] 段胜钢,齐辰,陆冬磊,等.2018—2020年上海市致泻大肠埃希氏菌感染的流行病学特征分析[J].中国食品卫生杂志,2022,34(4):773-778.
[3] 曾永刚,王仲贵,黄进,等.四川省农村生活饮用水水质安全隐患及控制策略研究[J].成都大学学报(自然科学版),2016,35(2):205-208.
[4] 孟洁,雍明媛,翟雪莉,等.2022—2018年我国突发生活饮用水污染事件回顾性分析[J].医学动物防治,2021,37(3):216-219.
[5] 中华人民共和国卫生部,中国国家标准化管理委员会.生活饮用水标准检验方法:GB/T 5750—2006[S].北京: 中国标准出版社,2006:3-16.
[6] 胡睿娟. 水质污染指示菌-总大肠菌群[J].山西科技,2015,30(5):148-150
[7] 王伟杰,孙婷婷,李雪,等.2017—2019 年辽宁省市售食品中食源性致病菌监测结果分[J].食品安全质量检测学报,2021,12(9):3522-3528.
[8] 王伟杰,孙婷婷,魏彤竹,等.2017—2018 年辽宁省食品中食源性致病微生物监测结果分析[J].食品安全质量检测学报,2020,11(22):8566-8571.
[9] 秦思,沈赟,周翌婧,等.2016年江苏省食源性疾病患儿中致泻大肠埃希氏菌毒力基因分布与耐药性特征[J].食品安全质量检测学报,2020,11(6):2019-2024.
[10] 黄涛,山珊,黄艳梅,等.大肠埃希氏菌的分型方法及其研究进展[J].微生物学通报,2020,47(3):892-902.
[11] 中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理局.食品安全国家标准,食品微生物学检验,致泻大肠埃希氏菌检验:GB/4789.6—2016[S].北京:中国国家标准出版社.2016:1-10.
[12] 李雪,张眉眉,马景宏,等.辽宁省食源性副溶血性弧菌PFGE分子分型及血清型、耐药谱[J].中国微生态学杂志,2017,12(9):3522-3528.
[13] ELAFIFY M,KHALIFA H O,AL-ASHMAWY M,et al.Prevalence and antimicrobial resistance of Shiga toxin-producing Escherichia coli in milk and dairy products in Egypt[J].J Environ Sci Health,2020,55(3):265-272.
[14] ANGELA H A M H,JANIEKE N J V,INGRID F,et al.Comparative genomics reveals a lack of evidence for pigeons as a main source of stx 2f-carrying Escherichia coli causing disease in humans and the common existence of hybrid Shiga toxin-producing and enteropathogenic E.coli pathotypes[J].BMC Genomics,2019,20(1):1-14.
[15] 万洁. 农村生活饮用水水质检测分析[J].农经管理,2022(16):121-124.
[16] 李双双,杜强,杜霞,等.湖库型饮用水水源地安全评价新方法探索[J].中国农村水利水电,2022(7):151-157.
[17] 黄曾,赵卫红,丁欣悦,等.2018年丰城市生活饮用水微生物监测结果分析及解决措施[J].中国当代医药,2021,28(15):189-191.
[18] 刘伟,白婧,尹可欣.2015—2019 年北京市海淀区食源性腹泻患者致泻大肠埃希氏菌型别分布和耐药性分析[J].现代检验医学杂志,2021,36(6):188-190.
[19] 杨蓉,李垒,霍晓芹,等.水质标准中指示微生物的发展及现状[J].中国环境监测,2020,36(4):1-9.
[20] 卢朝虹,谭斌,魏科.2016—2020年重庆市渝北区生活饮用水水质监测结果分析[J].现代医药卫生,2022,38(2):341-344.
[21] 罗赟,何玲玲,刘颜.2019年绵阳市生活饮用水水质监测结果分析[J].环境卫生学杂志,2020,10(5):454-459.
[22] 詹永国,余玉文,胡俊贤,等.2017年黄山市农村集中式供水水质监测[J].职业与健康,2018,34(15):2138-2140.
[23] 彭敏. 2015—2019年淮北市生活饮用水水质监测结果[J].职业与健康,2020,36(11):1534-1537.
[24] 安伟,桑晨惠,陈文秀,等.基于风险评估的饮用水水质标准制定方法探究[J].给水排水,2022,48(8):1-5.
[25] 刘雪莹,朱元,陈海平,等.2016—2017 年北京市某区生活饮用水卫生应急监测结果[J].职业与健康,2018,34(23):3266-3269.