Study on hepatoprotective effect of total honeysuckle saponins on lead poisoning rats based on NF-κB/IL-1β pathway

Abstract

Abstract: Objective To investigate the hepatoprotective effect of total saponins of honeysuckle on lead poisoning rats based on nuclear factor kappa-B（NF-κB）/interleukin-1β（IL-1β） pathway. Methods 100 clean SD rats were divided into normal control group,model control group,low,medium and high dose groups of total honeysuckle saponins. Except the normal control group,the other groups were injected with lead acetate solution（10 mg/kg） for four weeks. During this period,the rats in the low,medium and high dose groups of total honeysuckle saponins were given the corresponding doses of total honeysuckle saponins by gavage every day,and the normal control group and the model control group were given the same volume of normal saline by gavage. After the test,the rat serum and liver were obtained,and the liver/body ratio,liver lead content,serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,gamma-glutamyltransferase（GGT）,malondialdehyde（MDA）,superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） levels were determined. The reverse transcription-polymerase chain reaction（RT-PCR） and Western blotting were used to determine liver NF-κB and IL-1β levels. Results Compared with the normal control group,the body weight,SOD and GSH-Px levels of the model control group decreased,liver/body ratio,liver lead content,serum AST[（256.32±16.24）vs（46.81±15.24）μ/L],ALT[（354.32±18.54）vs（35.36±15.85）μ/L],GGT level[（5.64±0.31）vs（0.94±0.24）μ/L],liver MDA level[（654.49±16.29）vs（266.54±15.52）ng/g],liver NF-κB and IL-1β mRNA protein expression levels[0.82±0.28 vs 3.61±0.23, 0.48±0.29 vs 3.71±0.32,0.49±0.12 vs 1.69±0.11,0.38±0.16 vs 1.98±0.14]increased（P<0.05）. Compared with the model control group,the body weight,SOD,GSH-Px levels in the low,medium and high dose groups of total saponins of honeysuckle decreased,serum AST[（88.32±15.74）vs（135.20±10.8）,（186.28±12.27）,（256.32±16.24）μ/L],ALT[（154.68±19.78）vs（204.28±20.47）,（257.68±19.44）,（354.32±18.54）μ/L],GGT level[（2.23±0.27）vs（3.27±0.34）,（4.73±0.27）,（5.64±0.31）μ/L],liver MDA level[（358.58±15.41）vs（485.52±10.59）,（588.69±12.53）,（654.49±16.29）ng/g],liver NF-κB and IL-1β mRNA protein expression levels decreased（3.61±0.23 vs 2.89±0.25,2.29±0.21,1.65±0.27,3.71±0.32 vs 2.93±0.30,2.33±0.29. 1.15±0.24,1.69±0.11 vs 1.39±0.10,0.95±0.14,0.74±0.13,1.98±0.14 vs 1.35±0.15,0.89±0.14,0.53±0.13）（P<0.05）. With the increase of total honeysuckle saponin dose,the body weight,SOD and GSH-Px levels of the low,medium and high dose groups of total honeysuckle saponins gradually increased,and the liver/body ratio,liver lead content,serum AST,ALT,GGT levels,liver MDA level,liver NF-κB,IL-1β mRNA protein expression levels gradually decreased,with a significant dose-effect relationship（P<0.05）. Conclusion The total honeysuckle saponins can significantly reduce liver oxidative damage and inflammatory response in lead poisoning rats,and have obvious hepatoprotective effects,its mechanism may be related to the inhibition of liver NF-κB/IL-1β pathway activation by total honeysuckle saponins in lead poisoning rats.

Key words: NF-κB, IL-1β, Total honeysuckle saponins, Lead, Liver protection

CLC Number:

R-332

HUANG Hua, ZHENG Chong, ZHANG Kai. Study on hepatoprotective effect of total honeysuckle saponins on lead poisoning rats based on NF-κB/IL-1β pathway. [J]OCCUPATION AND HEALTH, 2023, 39(5): 620-625.

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