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Effect of occupational nickel exposure on malignant biological behavior of colorectal cancer SW620 cells:Based on the miR-101-5p-ESR1 pathway
- WANG Xing, ZHANG Biao
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2024, 40(14):
1904-1910.
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Objective Based on microRNA-101-5p(miR-101-5p)-estrogen receptor(ESR1) pathway,to explore the effect of occupational nickel exposure on malignant biological behavior of colorectal cancer SW620 cells. Methods The colorectal cancer SW620 cell group,low-dose,medium-dose,and high-dose groups exposed to nickel(NiSO4 final concentrations were 200.0,400.0,and 800.0 mg/mL) were set up in an environment of 37 ℃,5% CO2,and 95% humidity.After culturing for 72 hours,the cell viability was measured by methyl thiazolyl tetrazolium(MTT) method,the number of single clones was measured by crystal violet staining,the cell apoptosis was detected by flow cytometry,the level of cell invasion and migration was measured by matrigel coating membrane and scratch test,and the levels of miR-101-5p and ESR1 were determined by real time fluorescence quantitative PCR and Western blot. Results Compared with the colorectal cancer SW620 cell group,the survival rate,colony formation number,membrane penetration number,migration distance,ESR1 mRNA protein expression level of low,medium and high dose NiSO4 exposure groups[(1.39±0.22)vs(1.91±0.31),(3.27±0.50),(5.89±0.47) and (0.45±0.07) vs(0.88±0.11),(1.25±0.13),(1.45±0.18)] increased,while the apoptosis rate,miR-101-5p expression level[(3.58±0.36) vs(2.85±0.21),(2.29±0.19),(1.15±0.08)] decreased,and the differences were statistically significant(all P<0.05).With the gradual increase of NiSO4 exposure dose level,the survival rate,colony formation number,membrane penetration number,migration distance,ESR1 mRNA protein expression level of each dose group exposed to NiSO4[(1.91±0.31) vs(3.27±0.50) vs(5.89±0.47),(0.88±0.11) vs(1.25±0.13) vs(1.45±0.18)] gradually increased,the apoptosis rate,miR-101-5p expression level[(2.85±0.21) vs(2.29±0.19) vs(1.15±0.08)] gradually decreased,and the differences were statistically significant(all P<0.05). Conclusion Nickel inhibits the expression of miR-101-5p in colorectal cancer cells and promotes the high expression of ESR1,thereby promoting the proliferation,invasion and migration of colorectal cancer cells,and inhibiting apoptosis.