GⅠ.P11-GⅠ.6型诺如病毒分子特征分析

职业与健康 ›› 2023, Vol. 39 ›› Issue (8): 1016-1020.

GⅠ.P11-GⅠ.6型诺如病毒分子特征分析

尹松月¹, 信振江¹, 秦萌¹, 高志勇², 张建军¹, 张玲¹

1.北京市丰台区疾病预防控制中心微生物检验科,北京 100071;
2.北京市疾病预防控制中心微生物检验科,北京 100013

收稿日期:2022-07-17 修回日期:2022-08-22 发布日期:2026-03-11
通信作者: 张玲,主任技师,E-mail：1239631337@qq.com
作者简介:尹松月,女,中级检验师,主要从事病原微生物检测工作。
基金资助:
中美新发再发传染病国际合作项目中国诺如病毒暴发网络实验室建立（1U01GH002224）

Analysis on molecular characterization of norovirus GⅠ.P11-GⅠ.6

YIN Song-yue¹, XIN Zhen-jiang¹, QIN Meng¹, GAO Zhi-yong², ZHANG Jian-jun¹, ZHANG Ling¹

1. Microbiological Testing Department,Beijing Fengtai District Center for Disease Control and Prevention,Beijing 100071,China;
2. Microbiological Testing Department,Beijing Center for Disease Control and Prevention,Beijing 100031,China

Received:2022-07-17 Revised:2022-08-22 Published:2026-03-11
Contact: ZHANG Ling,Chief technician,E-mail：1239631337@qq.com

摘要/Abstract

摘要： 目的分析引起北京市丰台区某小学1起胃肠炎疫情的诺如病毒分型特征,为诺如病毒防控提供参考依据。方法 2021年10月在北京市丰台区某小学发生1起胃肠炎疫情,留取粪便标本做便常规检测和便隐血检测,并在便标本中提取核酸,使用荧光定量PCR技术进行诺如病毒核酸检测,采用一步法逆转录PCR扩增特异性核酸片段分析诺如病毒亚型。结果共14例胃肠炎病例,14份粪便标本便常规结果正常,7份便隐血结果阳性,阳性率为50.0%。13份标本检测出诺如病毒阳性,基因型别为GⅠ.P11-GⅠ.6。结论本次疫情检测出的诺如病毒型别引起的胃肠炎便隐血阳性率较高,超过其他文献报道的比率,并发现其在氨基酸水平上聚合酶区和VP1区都发生了关键位点的变异。需持续进行疫情监测和防控工作,以减少该类疫情的发生。

关键词: 诺如病毒, 便隐血, 型别

Abstract: Objective To analyze the genotyping characteristics of norovirus which caused gastroenteritis epidemic in a primary school in Fengtai District of Beijing,provide scientific reference for the prevention and control of norovirus. Methods In October 2021,a gastroenteritis epidemic occurred in a primary school in Fengtai District of Beijing. The stool samples were collected for routine stool test and occulted blood test,nucleic acid was extracted from the samples and detected by fluorescence quantitative PCR,and one-step reverse transcription PCR was used to amplify specific nucleic acid fragments and analyze norovirus subtypes. Results There were 14 cases of gastroenteritis,all specimens had normal routine stool results,seven specimens were positive in occulted blood test,with the positive rate of 50.0%. 13 norovirus positivecases were detected,and the genotype was GⅠ.P11-GⅠ.6. Conclusions The positive rate of fecal occult blood in gastroenteritis caused by norovirus type detected in this epidemic is high,which is higher than that reported in other literature. There are key site mutations in the polymerase area and VP1 area at the amino acid level. In order to reduce the occurrence of the epidemic,continuous epidemic monitoring and prevention and control work should be carried out.

Key words: Norovirus, Occulted blood test, Subtype

中图分类号:

尹松月, 信振江, 秦萌, 高志勇, 张建军, 张玲. GⅠ.P11-GⅠ.6型诺如病毒分子特征分析. [J]职业与健康, 2023, 39(8): 1016-1020.

YIN Song-yue, XIN Zhen-jiang, QIN Meng, GAO Zhi-yong, ZHANG Jian-jun, ZHANG Ling. Analysis on molecular characterization of norovirus GⅠ.P11-GⅠ.6. [J]OCCUPATION AND HEALTH, 2023, 39(8): 1016-1020.

参考文献

[1] SELL J,DOLAN B.Common Gastrointestinal Infections[J].Prim Care,2018,45(3):519-532.
[2] SADKOWSKA-TODYS M,ZIELINSKI A,CZARKOWSKI M P.Infectious diseases in Poland in 2016[J].Przegl Epidemiol,2018,72(2):129-141.
[3] AHMED S M,HALL A J,ROBINSON A E,et al.Global prevalence of norovirus in cases of gastroenteritis:A systematic review and meta-analysis[J].Lancet Infect Dis,2014,14(8):725-730.
[4] ROBILOTTI E,DERESINSKI S,PINSKY B A.Norovirus[J].Clin Microbiol Rev,2015,28(1):134-164.
[5] BONYAI K,ESTES M K,MARTELLA V,et al.Viral gastroenteritis[J]. Lancet,2018,392(10142):175-186.
[6] CHEN Y F E,WANG C Y,CHIU C H,et al.Molecular epidemiology and clinical characteristics of norovirus gastroenteritis with seizures in children in Taiwan,2006-2015[J].Medicine(Baltimore),2019,98(40):e17269.
[7] INNS T,HARRIS J,VIVANCOS R,et al.Community-based surveillance of norovirus disease:A systematic review[J].Meta-Analysis BMC Infect Dis,2017,17(1):657.
[8] JIANG X,WAN G M,GRAHAM D Y,et al.Expression,self-assembly,and antigenicity of the norwalk virus capsid protein[J].Virol,1992, 66(11):6527-6532.
[9] ATMAR R L,RAMANI S,ESTES M K.Human noroviruses:recent advances in a 50-year history[J].Curr Opin Infect Dis,2018,31(5):422-432.
[10] CHHABRA P,DE GRAAF M,PARRA G I,et al.Updated classification of norovirus genogroups and genotypes[J].J Gen Virol,2019 100(10):1393-1406.
[11] 沈玲羽,姜博,严寒秋,等.诺如病毒基因分型研究进展[J].中华流行病学杂志,2020,41(11):1927-1932.
[12] KRONEMAN A,VEGA E,VENNEMA H,et al.Proposal for a unified norovirus nomenclature and genotyping[J].Arch Virol,2013,158(10):2059-2068.
[13] ANDO T,NOEL J S,FANKHAUSER R L.Genetic classification of “Norwalk-like viruses”[J].J Infect Dis,2000,181(Suppl 2):S336-348.
[14] GAO Z Y,LIU B W,HUO D,et al.Increased norovirus activity was associated with a novel norovirus GII.17 variant in Beijing,China during winter 2014-2015[J].BMC Infect Dis,2015:574.
[15] VINJE J.Advances in laboratory methods for detection and typing of norovirus[J].J Clin Microbiol,2015,53(2):373-381.
[16] CANNON J L,BARCLAY L,COLLINS N R,et al.Genetic andepidemiologic trends of Norovirus outbreaks in the United States from 2013 to 2016 demonstrated emergence of Novel G II.4 recombinant viruses[J].J Clin Microbiol,2017,55(7):2208-2221.
[17] 俞进存,向以斌,靳淼.2015—2018年玉溪市5岁以下儿童诺如病毒分子流行病学分析[J].病毒学报,2020,36(3):446-454.
[18] DEVAL J,JIN Z,CHUANG Y C,et al.Structure(s),function(s),and inhibition of the RNA-dependent RNA polymerase of noroviruses[J].Virus Res,2017,15(234):21-33.
[19] SHIOTA T,OKAME M,TAKANASHI S,et al.Characterization of a Broadly Reactive Monoclonal Antibody against Norovirus Genogroups I and II:Recognition of a Novel Conformational Epitope[J].J Virol,2007,81(22):12298-12306.
[20] MIYAZAKI N,TAYLOR D W,HANSMAN G S,et al.Antigenic and cryo-electron microscopy structure analysis of a chimeric sapovirus capsid[J].J Virol,2015,90(5):2664-2675.
[21] GAO Z,LIU B,YAN H,et al.Norovirus outbreaks in Beijing,China,from 2014 to 2017[J].J Infect,2019,79(2):159-166.
[22] KIRBY A E,STREBY A,MOE C L.Vomiting as a symptom and transmission risk in norovirus illness:evidence from human challenge studies[J].PLoS One,2016,11(4):e0143759.
[23] CHEN S Y,FENG Y,CHAO H C,et al.Emergence in Taiwan of novel norovirus GII.4 variants causing acute gastroenteritis and intestinal haemorrhage in children[J].J Med Microbiol,2015,64(Pt 5):544-550.
[24] ZAMYATKIN D F,PARRA F,ALONSO J M M,et al.Structural insights into mechanisms of catalysis and inhibition in norwalk virus polymerase[J].J Biol Chem,2008,283(12):7705-7712.
[25] PARRA G I,SOSNOVTSEV S V,ABENTE E J,et al.Mapping and modeling of a strain-specific epitope in the Norwalk virus capsid inner shell[J].Virology,2016,492:232-241.
[26] BOK K,PARRA G I,MITRA T,et al.Chimpanzees as an animal model for human norovirus infection and vaccine development[J].Proc Natl Acad Sci USA,2011,108(1):325-330.