关键词: 结核分枝杆菌, 耐药基因, 特征

Abstract: Objective To analyze the characteristics of resistance mutations in genes rpoB for rifampin（RFP）,katG,inh A and aphC for isoniazid（INH） in clinical isolates of mycobacterium tuberculosis complex（MTBC）,and provide theoretical basis for formulating precise treatment plans for drug-resistant tuberculosis patients. Methods From October 2021 to January 2024,712 MTBC isolates from tuberculosis patients in Tianjin Center for Tuberculosis Control were collected,and the drug-resistant mutations were detected by fluorescence PCR melting curve method. Results Among 712 strains of MTBC,a total of 156 strains with drug-resistant gene mutations were detected. Among them,88 strains had mutations in the rpoB gene,86 strains had mutations in the katG315 gene site,40 strains had mutations in the inhA gene,and 9 strains had mutations in the aphC gene. Among the strains with mutations resistant to PFP molecules,a total of 9 mutation types were detected,mainly characterized by single point mutations,accounting for 93.18%. Among them,rpoB529-533 was the most important mutation site,accounting for 59.09%. Among the strains with mutations resistant to INH molecules,a total of 8 mutation types were detected,mainly characterized by single point mutations,accounting for 96.24%. Among them,the most important mutation was katG315,accounting for 62.41%. Conclusion MTBC has a diversity of mutation types related to RIF and INH resistance,with a predominance of single point mutations,mainly in the rpoB and katG315 gene mutation sites. Deep analysis of the mutation characteristics of MTBC resistance-related genes lays a foundation for further understanding the production mechanism of drug resistance,and also provides an important reference for subsequent drug resistance prevention,control,and treatment.

Key words: Mycobacterium tuberculosis, Drug resistance gene, Characteristics