关键词: 食品, 唐菖蒲伯克霍尔德菌, 椰毒假单胞菌米面亚种, 米酵菌酸, 污染

Abstract: Objective To investigate the contamination of Burkholderia galdioli in six food samples,namely,wet wide rice noodles,wet rice noodles,wet white fungus,dried white fungus,wet wood ear,and dried wood ear,collected from Longgang District of Shenzhen City,and to explore the distribution of the contamination. Methods From April to October 2023,a total of 103 samples were randomly collected from supermarkets and farmers' markets located in Longgang District of Shenzhen. Bacterial isolation and identification procedures were conducted in strict accordance with GB 4789.29-2020 "National food safety standard,Food microbiological examination,Burkholderia galdioli（Pseudomonas cocovenenans subsp. farinofermentans）". Real-time fluorescence PCR was employed to detect the toxin-producing bon gene of Pseudomonas cocovenenans subsp. farinofermentans. Furthermore,the detection of bongkrekic acid in the samples was performed in accordance with GB 5009.189-2023 "National food safety standard,Determination of bongkrekic acid in food". Results A total of thirty-eight strains of Burkholderia galdioli were cultured from 103 samples,with a positive rate of 33.98%,three of which were specifically identified as the subspecies Pseudomonas cocovenenans subsp. Farinofermentans,with a positive rate of 2.91%. Conclusion Burkholderia galdioli（Pseudomonas cocovenenans subsp. farinofermentans） was detected in wet rice noodles and dried wood ear samples,indicating that there was a risk of contamination in wet rice noodles and dried wood ear by Burkholderia galdioli（Pseudomonas cocovenenans subsp. farinofermentans） in Longgang District of Shenzhen. To minimize or eliminate the potential hazards associated with this risk,relevant departments should strengthen their supervision of food safety and ensure the protection of public health and dietary safety.

Key words: Food, Burkholderia galdioli, Pseudomonas cocovenenans subsp. farinofermentans, Bongkrekic acid, Contamination