铁皮石斛等8种药食同源中药材中总汞含量的过氧化氢辅助微波消解-顺序注射冷原子荧光光谱测定法

职业与健康 ›› 2025, Vol. 41 ›› Issue (1): 42-45.

铁皮石斛等8种药食同源中药材中总汞含量的过氧化氢辅助微波消解-顺序注射冷原子荧光光谱测定法

韦丽丽, 覃月媚, 石莉莉

梧州市疾病预防控制中心理化检验科,广西梧州 543002

收稿日期:2024-04-29 修回日期:2024-05-13 出版日期:2025-01-01 发布日期:2025-12-17
作者简介:韦丽丽,女,副主任技师,主要从理化检验工作。

Determination of total Hg content in 8 medicinal and edible Chinese medicinal materials including dendrobium officinale by hydrogen peroxide assisted microwave digestion sequential injection cold atomic fluorescence spectrometry

WEI Lili, QIN Yuemei, SHI Lili

Physical and Chemical Department Laboratory,Wuzhou Center for Disease Control and Prevention,Wuzhou,Guangxi 543002,China

Received:2024-04-29 Revised:2024-05-13 Online:2025-01-01 Published:2025-12-17

摘要/Abstract

摘要： 目的建立过氧化氢辅助微波消解-顺序注射冷原子荧光光谱法测定铁皮石斛等8种药食同源中药材中的总汞含量。方法采用硝酸-过氧化氢体系,微波消解仪消解中药材样品,并对消解体系、微波程序、汞灯预热方式、载气和屏蔽气流量、载流和硼氢化钾浓度等实验条件进行优化。结果在最佳实验条件下,汞的测定范围为0~1.0 μg/L,线性回归方程为Y=1 586.13X-21.93,回归方程相关系数（r）为0.999 8,检出限为0.011 μg/L,相对标准偏差（relative standard deviation,RSD）为1.34%,加标回收率为92.4%~102.4%。结论该方法操作简单,准确度好,精密度高,可方便测定批量中药材中的总汞含量。

关键词: 微波消解, 过氧化氢, 冷原子荧光光谱法, 汞, 药食同源中药材

Abstract: Objective To develop a method of hydrogen peroxide microwave digestion and sequential injection cold-atomic fluorescence spectrometry for the determination of Hg content in 8 medicinal and edible Chinese medicinal herbs including dendrobium officinale. Methods The Chinese medicinal materials samples were digested by microwave digester using nitric acid-hydrogen peroxide system,and the digestion system,microwave program,mercury lamp preheating,carrier gas and shielding gas flow,carrier current and potassium borohydride concentration were studied. Results Under the conditions proposed,the linear range was 0-1.0 μg/L,the linear regression equation was Y=1 586.13X-21.93,the correlation coefficient（r） was 0.999 8,the detection limit of Hg was 0.011 μg/L,the relative deviation was 1.34% and the recovery was 92.4%-102.4%. Conclusion The method is simple,and has good accuracy and hight sensitivity. It can be easily used for the determination of Hg in Chinese medicinal materials.

Key words: Microwave digestion, Hydrogen peroxide, Cold-atomic fuorescence spectrometry, Hg, Medicinal and edible Chinese medicinal herbs

中图分类号:

R115

韦丽丽, 覃月媚, 石莉莉. 铁皮石斛等8种药食同源中药材中总汞含量的过氧化氢辅助微波消解-顺序注射冷原子荧光光谱测定法. [J]职业与健康, 2025, 41(1): 42-45.

WEI Lili, QIN Yuemei, SHI Lili. Determination of total Hg content in 8 medicinal and edible Chinese medicinal materials including dendrobium officinale by hydrogen peroxide assisted microwave digestion sequential injection cold atomic fluorescence spectrometry. [J]OCCUPATION AND HEALTH, 2025, 41(1): 42-45.

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