2023年大连市基于致病菌识别网的细菌性传染病监测结果分析

职业与健康 ›› 2025, Vol. 41 ›› Issue (5): 626-630.

2023年大连市基于致病菌识别网的细菌性传染病监测结果分析

陈玉凤, 于鹏, 侯君, 周林, 栾明春, 岳雪瑶

大连市疾病预防控制中心微生物检验科,辽宁大连 116033

收稿日期:2024-06-16 修回日期:2024-07-02 发布日期:2025-12-17
通信作者: 岳雪瑶,主管技师,E-mail：756572358@qq.com
作者简介:陈玉凤,女,主任技师,主要从事细菌和寄生虫检验工作。
基金资助:
2023年大连市疾病预防控制中心预防医学科技攻关计划项目（DLCDC-11）; 大连市医学科学研究计划项目（2311030）

Analysis on monitoring results of bacterial infectious disease based on pathogenic bacteria identification network in Dalian City in 2023

CHEN Yufeng, YU Peng, HOU Jun, ZHOU Lin, LUAN Mingchun, YUE Xueyao

Microbial Testing Department,Dalian Center for Disease Control and Prevention,Dalian,Liaoning 116033,China

Received:2024-06-16 Revised:2024-07-02 Published:2025-12-17
Contact: YUE Xueyao,Technician in charge,E-mail：756572358@qq.com

摘要/Abstract

摘要： 目的了解2023年大连市基于致病菌识别网的细菌性传染病监测中细菌分离情况及其血清型、毒力基因和药物敏感性检测结果。方法 2023年大连市按照《国家致病菌识别网实验室监测技术手册》（2022试用版）中的方法进行相关细菌分离鉴定;参照GB 4789.4—2016《食品安全国家标准食品微生物学检验沙门氏菌检验》,采用玻片凝集法进行沙门菌血清分型;按照《副溶血性弧菌tlh/tdh/trh三重实时荧光PCR检测试剂盒》说明书,采用荧光定量PCR法检测副溶血性弧菌毒力基因;按照《Sensititre^TM革兰阴性菌MIC药敏板》使用说明书,采用微量肉汤法检测副溶血性弧菌、沙门菌和致泻大肠埃希菌药物敏感性。结果 2023年大连市共监测三大类、5类样品765份,其中腹泻相关样品445份,检出率最高的细菌是副溶血性弧菌（22.25%）;呼吸道相关样品258份,包括呼吸道-百日咳相关样品38份,检出百日咳杆菌2株（5.26%）,呼吸道相关-肺炎样品98份,检出率最高的是肺炎克雷伯杆菌（27.55%）,呼吸道相关-其他样品122份,检出金黄色葡萄球菌和枸橼酸杆菌属各1株;自然疫源相关样品62份,检出4株布鲁杆菌（6.45%）。13株沙门菌共鉴定出 10 种血清型,没有优势血清型,布利丹沙门菌和肠炎沙门菌略多。99株副溶血性弧菌tlh、tdh和trh检出率分别为100.00%、77.78%和0。副溶血性弧菌、沙门菌、致泻大肠埃希菌均有不同程度耐药,沙门菌耐药情况最为严重,有10株菌对3种以上抗生素耐药,4株副溶血性弧菌对碳青霉烯类抗生素耐药。结论不同地区传染病病原菌构成不同,菌株特征也具有差异性,对感染性疾病进行细菌分离鉴定,并对分离到的细菌进行血清型、毒力基因、药物敏感性等菌株特征检测意义重大。

关键词: 致病菌识别网, 细菌性传染病, 血清型, 毒力基因, 药物敏感性

Abstract: Objective To understand the bacterial isolation situation,serotype,virulence genes,and drug sensitivity test results in the monitoring of bacterial infectious diseases based on the pathogenic bacteria identification network in Dalian City in 2023. Methods In 2023,relevant bacterial isolation and identification were carried out in Dalian City according to the methods in the Laboratory monitoring technical manual of the national pathogenic bacteria identification network（2022 trial edition）. According to GB 4789.4-2016 National food safety standard for microbiological examination of food-Salmonella test,the serum typing of Salmonella was carried out using the slide agglutination method. According to the instructions of the Triple real-time fluorescence PCR detection kit for Vibrio parahaemolyticus tlh/tdh/trh,fluorescence quantitative PCR method was used to detect the virulence genes of Vibrio parahaemolyticus. According to the user manual of Sensititre^TM Gram negative bacteria MIC drug sensitivity board,the drug sensitivity of Vibrio parahaemolyticus,Salmonella,and Escherichia coli causing diarrhea was detected using the broth microdilution method. Results In 2023,a total of 765 samples from five kinds of three categories were monitored in Dalian City. Among 445 samples related to diarrhea,the highest detection rate was Vibrio parahaemolyticus（22.25%）. There were 258 respiratory infection related samples,including 38 pertussis related samples,two strains of Bordetella pertussis were detected（5.26%）. Among 98 respiratory related-pneumonia samples,the highest detection rate was Klebsiella pneumoniae（27.55%）. Among 122 respiratory related- other samples,one strain of Staphylococcus aureus and one strain of Citrobacter were detected. Out of 62 samples related to natural epidemics,four strains of Brucella were detected（6.45%）. 13 strains of Salmonella were identified,with a total of 10 serotypes,without any dominant serotypes. Salmonella bredan and Salmonella enteritis were slightly more common. The detection rate of tlh,tdh and trh for 99 strains of Vibrio parahaemolyticus was 100.00%,77.78% and 0,respectively. Vibrio parahaemolyticus, Salmonella,and Escherichia coli causing diarrhea all had varying degrees of resistance,with Salmonella showing the most severe resistance.10 strains of bacteria were resistant to three or more antibiotics,and four strains of Vibrio parahaemolyticus were resistant to carbapenems. Conclusion The composition of infectious disease pathogens in different regions is different,and the characteristics of strains are also different,and it is of great significance to isolate and identify bacteria from infectious diseases,and to detect the serotype,virulence gene and drug sensitivity of the isolated bacteria.

Key words: Pathogenic bacteria identification network, Bacterial infectious diseases, Serotypes, Toxicity genes, Drug sensitivity

中图分类号:

R117

陈玉凤, 于鹏, 侯君, 周林, 栾明春, 岳雪瑶. 2023年大连市基于致病菌识别网的细菌性传染病监测结果分析. [J]职业与健康, 2025, 41(5): 626-630.

CHEN Yufeng, YU Peng, HOU Jun, ZHOU Lin, LUAN Mingchun, YUE Xueyao. Analysis on monitoring results of bacterial infectious disease based on pathogenic bacteria identification network in Dalian City in 2023. [J]OCCUPATION AND HEALTH, 2025, 41(5): 626-630.

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