长链非编码RNA CUST-49525和CUST-40243在1，4-苯醌致K562细胞毒性中的表达

职业与健康 ›› 2023, Vol. 39 ›› Issue (1): 9-14.

长链非编码RNA CUST-49525和CUST-40243在1，4-苯醌致K562细胞毒性中的表达

刘晓东, 黄云飞, 田薇

新疆医科大学公共卫生学院，新疆乌鲁木齐 830011

收稿日期:2022-06-24 修回日期:2022-07-04 发布日期:2026-02-28
通信作者: 田薇，副教授，E-mail：wei4148@163.com
作者简介:刘晓东，男，在读硕士研究生，研究方向为遗传与分子毒理学。
基金资助:
新疆维吾尔自治区高校科研计划（XJEDU2020Y023）; 新疆维吾尔自治区“十四五”高等学校特色学科——公共卫生与预防医学

Expression of long noncoding RNA CUST-49525 and CUST-40243 in K562 cytotoxicity induced by 1，4-benzoquinone

LIU Xiao-dong, HUANG Yun-fei, TIAN Wei

School of Public Health, Xinjiang Medical University, Urumqi Xinjiang 830011, China

Received:2022-06-24 Revised:2022-07-04 Published:2026-02-28
Contact: TIAN Wei，Associate professor，E-mail：wei4148@163.com

摘要/Abstract

摘要： 目的通过检测LncRNA CUST-49525和CUST-40243在1，4-苯醌致细胞毒性过程中表达的变化，为后期研究两者在细胞毒性中参与的调控作用提供依据。方法使用0、10、20、40 μmol/L的1，4-苯醌染毒K562细胞24 h，光学显微镜下观察细胞形态特征;采用CCK-8法测定细胞生长情况;采用流式细胞术分析细胞周期分布和凋亡情况;使用实时荧光定量PCR法测定LncRNA CUST-49525和CUST-40243表达情况。结果 1，4-苯醌染毒K562细胞24 h后镜下观察细胞数量和形态发生明显改变，与对照组相比，20和40 μmol/L剂量组K562细胞相对增殖率显著降低（P<0.01）;各剂量组1，4-苯醌均可诱导细胞发生凋亡，呈现浓度依赖性（P<0.01），与对照组相比，20和40 μmol/L剂量组K562细胞G1期比例增加（P<0.01），S期比例下降（P<0.05）;实时荧光定量PCR结果显示，与对照组相比，20和40 μmol/L剂量组K562细胞中LncRNA CUST-49525和CUST-40243表达量增加（P<0.01）。结论 LncRNA CUST-49525和CUST-40243在1，4-苯醌致K562细胞毒性过程中异常表达，两者可能参与调控细胞毒性过程，但其具体作用机制还需要进一步研究。

关键词: 1，4-苯醌, 细胞毒性, 长链非编码RNA

Abstract: Objective To detect the expression changes of LncRNA CUST-49525 and CUST-40243 in the process of cytotoxicity induced by 1，4-benzoquinone，provide the basis for the later study of their regulatory role in cytotoxicity. Methods K562 cells were exposed to 0，10，20 and 40 μmol/L 1，4-benzoquinone for 24 hours，and the morphological characteristics of the cells were observed under optical microscope. CCK-8 method was used to measure the cell growth. Cell cycle distribution and apoptosis were analyzed by flow cytometry. The expression of LncRNA CUST-49525 and CUST-40243 was measured by real-time fluorescence quantitative PCR. Results After K562 cells were exposed to 1，4-benzoquinone for 24 hours，the number and morphology of K562 cells were obviously changed under microscope. Compared with the control group，the relative proliferation rate of K562 cells in 20 and 40 μmol/L dosage groups decreased significantly（P<0.01）. 1，4-benzoquinone in each dose group could induce cell apoptosis in a concentration-dependent manner（P<0.01）. Compared with the control group，the G1 phase proportion of K562 cells in the 20 and 40 μmol/L dosage groups increased（P<0.01），while the S phase proportion decreased（P<0.05）. The results of real-time quantitative PCR showed that compared with the control group，the expression levels of LncRNA CUST-49525 and CUST-40243 in K562 cells in 20 and 40 μmol/L dosage groups increased（P<0.01）. Conclusion LncRNA CUST-49525 and CUST-40243 are abnormally expressed in the cytotoxicity of K562 cells induced by 1，4-benzoquinone，and they may be involved in the regulation of cytotoxicity，but their specific mechanism needs further study.

Key words: 1，4-benzoquinone, Cytotoxicity, Long noncoding RNA

中图分类号:

R114

刘晓东, 黄云飞, 田薇. 长链非编码RNA CUST-49525和CUST-40243在1，4-苯醌致K562细胞毒性中的表达. [J]职业与健康, 2023, 39(1): 9-14.

LIU Xiao-dong, HUANG Yun-fei, TIAN Wei. Expression of long noncoding RNA CUST-49525 and CUST-40243 in K562 cytotoxicity induced by 1，4-benzoquinone. [J]OCCUPATION AND HEALTH, 2023, 39(1): 9-14.

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