枸杞多糖对睡眠剥夺大鼠血液中T细胞亚群及相关炎性因子的影响

职业与健康 ›› 2023, Vol. 39 ›› Issue (3): 317-321.

枸杞多糖对睡眠剥夺大鼠血液中T细胞亚群及相关炎性因子的影响

栾和芝¹, 单铁强², 姜柳², 韩长海², 祁皎², 吕晶³

1.河北港口集团有限公司港口医院检验科，河北秦皇岛 066000;
2.秦皇岛市海港医院检验科，河北秦皇岛 066000;
3.河北工程大学医学院，河北邯郸 056002

收稿日期:2022-06-24 修回日期:2022-07-04 发布日期:2026-03-02
通信作者: 单铁强，副主任技师，E-mail：sty751218@163.com
作者简介:栾和芝，女，主管技师，主要从事检验学的临床与研究工作。

Effects of Lycium barbarum polysaccharides on T cell subtypes and related inflammatory factors in blood of sleep deprived rats

LUAN He-zhi¹, SHAN Tie-qiang², JIANG Liu², HAN Chang-hai², QI Jiao², LYU Jing³

1. Laboratory Department，Port Hospital of Hebei Port Group Co.,Ltd，Qinhuangdao Hebei 066000，China;
2. Department of Laboratory，Qinhuangdao Haigang Hospital，Qinhuangdao Hebei 066000，China;
3. Medical College，Hebei Engineering University，Handan Hebei 056002，China

Received:2022-06-24 Revised:2022-07-04 Published:2026-03-02
Contact: SHAN Tie-qiang，Deputy chief technician，E-mail：sty751218@163.com

摘要/Abstract

摘要： 目的分析枸杞多糖（Lycium barbarum polysaccharides，LBP）对睡眠剥夺大鼠血液中T细胞亚群型的数量、相关炎性因子以及大鼠行为能力的影响，探讨LBP对睡眠剥夺大鼠的免疫保护机制。方法将SD大鼠通过“多平台水环境”技术成功制备剥夺睡眠大鼠模型，按照干预方法将其均等分成3组（模型组、LBP干预组及西药干预组），每组6只，同时把6只健康同种属大鼠作为对照组。在建模成功后4组鼠每天的干预用药不同，LBP干预组：灌服LBP（0.54 mg/kg），西药干预组：灌服舒乐安定（6 g/kg），模型组和对照组：灌服等容量三蒸水，持续用药4周。检测血液中T淋巴细胞亚群CD3⁺ T细胞、CD4⁺ T细胞、CD8⁺ T细胞的比例以及CD4⁺ T/CD8⁺ T的比值;ELISA 检测血液中肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、γ-干扰素（interferon-γ，IFN-γ）、白介素-1β（interleukin-1β，IL-1β）、白介素-2（interleukin-2，IL-2）及白介素-6（interleukin-6，IL-6）的含量;检测各组大鼠的行为学指标以衡量其大脑学习记忆功能的变化。结果和对照组相比，模型组大鼠血液中CD4⁺ T细胞比例减少[（25.64±2.53）%]，CD8⁺ T细胞比例增多[（20.71±2.21）%]，CD4⁺ T/CD8⁺ T比值（1.66±0.13）降低，血液中的炎性因子：TNF-α[（15.99±2.02）ng/mL]、IFN-γ[（3.22±0.45）ng/mL]、IL-1β[（22.70±2.87）pg/mL]、IL-2[（15.85±1.77）ng/mL]及IL-6[（64.65±6.23）pg/mL]的含量均增加，大鼠的行为能力[（42.36±4.60）s，（10.26±1.68）次]升高（均P<0.01）;和模型组相比，LBP干预组大鼠血液中CD4⁺ T细胞比例升高[（35.63±3.00）%]、CD8⁺ T细胞比例降低[（14.10±1.94）%]，CD4⁺ T/CD8⁺ T比值（2.38±0.21）增加，血液中的炎性因子：TNF-α[（10.25±1.72）ng/mL]、IFN-γ[（2.13±0.34）ng/mL]、IL-1β[（16.26±1.80）pg/mL]、 IL-2[（11.90±1.50）ng/mL]及IL-6[（46.74±4.39）pg/mL]的含量均下降，大鼠的行为能力[（31.51±3.98）s，（16.90±2.72）次]降低（均P<0.05）;LBP干预组与西药干预组相比，上述指标均没有明显差异（均P>0.05）。结论 LBP能够通过调节大鼠血液中T细胞亚型的数量及降低炎性因子的含量来提高睡眠剥夺大鼠的行为能力。

关键词: 枸杞多糖, 睡眠剥夺, T细胞亚群, 炎性因子, 行为能力

Abstract: Objective To analyze the effects of Lycium barbarum polysaccharide（LBP） on the number of T cell subtypes，related inflammatory factors and behavioral ability of sleep deprived rats，explore the immune protective mechanism of LBP on sleep deprived rats. Methods SD rats were successfully prepared into sleep deprivation rat models by "multiplatform water environment" technology. According to the intervention methods，they were equally divided into three groups（model group，LBP intervention group and western medicine intervention group） with six rats in each group，at the same time，six healthy rats of the same genus were used as the control group. After successful modeling，the intervention drugs of the four groups were different every day. LBP intervention group was given LBP（0.54 mg/kg） orally，western medicine intervention group was given estazolam（6 g/kg） orally，and model group and control group were given equal volume three distilled water orally，continuous medication for four weeks. The number of T lymphocyte subsets CD3⁺ T cells，CD4⁺ T cells，and the ratio of CD4⁺ T/CD8⁺ T in the blood were detected. The contents of tumor necrosis factor-α（TNF-α），γ-interferon（IFN-γ），interleukin-1β（IL-1β），interleukin-2（IL-2） and interleukin-6（IL-6） in blood. The behavioral indicators of rats in four groups were tested to measure the changes of brain learning and memory function. Results Compared with the control group，the percentage of CD4⁺ T cells in the blood of model group decreased[（25.64±2.53）%]，the percentage of CD8⁺ T cells increased[（20.71±2.21）%]，the ratio of CD4⁺ T/CD8⁺ T decreased（1.66±0.13），the contents of the inflammatory factor in the blood increased，which included TNF-α[（15.99±2.02）ng/mL]，IFN-γ[（3.22±0.45）ng/mL]，IL-1β[（22.70±2.87）pg/mL]，IL-2[（15.85±1.77）ng/mL] and IL-6[（64.65±6.23）pg/mL]，and the behavioral ability of rats[（42.36±4.60）s，（10.26±1.68）times] increased（all P<0.01）. Compared with the model group，the percentage of CD4⁺ T cells in the blood of LBP intervention group increased[（35.63±3.00）%]，the percentage of CD8⁺ T cells decreased[（14.10±1.94）%]， the ratio of CD4⁺ T/CD8⁺ T increased（2.38±0.21），the contents of the inflammatory factor in the blood decreased，which included TNF-α [（10.25±1.72）ng/mL]，IFN-γ[（2.13±0.34）ng/mL]，IL-1β[（16.26±1.80）pg/mL]，IL-2[（11.90±1.50）ng/mL] and IL-6[（46.74±4.39）pg/mL]，and the behavioral ability of rats[（31.51±3.98）s，(16.90±2.72）times] decreased（all P<0.05）. LBP intervention group and western medicine intervention group had not significant difference in the above indexes（all P>0.05）. Conclusion Lycium barbarum polysaccharide can improve the behavioral ability of sleep deprived rats by regulating the number of T cell subtypes and reducing the content of inflammatory factors in blood.

Key words: Lycium barbarum polysaccharide, Sleep deprivation, T cell subtypes, Inflammatory factors, Behavioral ability

中图分类号:

R-332

栾和芝, 单铁强, 姜柳, 韩长海, 祁皎, 吕晶. 枸杞多糖对睡眠剥夺大鼠血液中T细胞亚群及相关炎性因子的影响. [J]职业与健康, 2023, 39(3): 317-321.

LUAN He-zhi, SHAN Tie-qiang, JIANG Liu, HAN Chang-hai, QI Jiao, LYU Jing. Effects of Lycium barbarum polysaccharides on T cell subtypes and related inflammatory factors in blood of sleep deprived rats. [J]OCCUPATION AND HEALTH, 2023, 39(3): 317-321.

参考文献

[1] 孙毅红,刘心朗,周丽丽,等.睡眠障碍发病机制及对神经退行性病变的影响[J].中国老年学杂志,2022,42(7):1763-1766.
[2] 张晓洋,李笑,吴方,等.失眠对认知功能的影响研究进展[J].新乡医学院学报,2022,39(3):285-288.
[3] 聂笠,苏增锋.慢性失眠与免疫功能相关性研究进展[J].中华神经科杂志,2022,55(1):77-80.
[4] 刘威,胡克.睡眠剥夺与多系统相关疾病关系的研究进展[J].医学综述,2021,27(23):4696-4701.
[5] 宋艳梅,张启立,崔治家,等.枸杞子化学成分和药理作用的研究进展及质量标志物的预测分析[J].华西药学志,2022,37(2):206-213.
[6] 苏保洲.枸杞子活性成分药理作用研究进展[J].江苏中医药,2022,54(3):78-81.
[7] 孟玲欣,范贵民,王景涛.改良多平台水环境法制作大鼠REM睡眠剥夺模型的研究[J].黑龙江医药科学,2013,36(2):99-100.
[8] 李敬强,王庆福,张璐,等.不同睡眠类型选择性注意在睡眠剥夺中内稳态与昼夜节律研究[J].生物医学工程学杂志,2022,39(2):248-256.
[9] 邵娜,殷昊,李亚冉,等.Suvorexant改善慢性睡眠剥夺小鼠学习记忆损害的机制探讨[J].山东大学学报(医学版),2022,60(4):17-23.
[10] 刘陶,郁婧,钱召强,等.两种睡眠剥夺模型对小鼠情绪和学习记忆的影响[J].陕西师范大学学报(自然科学版),2018,46(5):77-82.
[11] 郭彤,王炳昕,王采玲,等.睡眠剥夺或束缚应激对小鼠刺激-反应/习惯记忆形成及提取的影响[J].国际药学研究杂志,2019,46(1):57-64.
[12] 刘鑫,单铁强,李伟,等.枸杞多糖对慢性睡眠剥夺大鼠大脑海马区5-羟色胺及其受体含量的影响[J].职业与健康,2021,37(18):2476-2479.
[13] 武学锋,单亚娟,李伟,等.枸杞多糖对慢性睡眠剥夺大鼠海马区去甲肾上腺素及其受体含量的影响[J].职业与健康,2021,37(3):338-341.
[14] 常媛媛,颜波,李榕.T细胞耗竭发生机制及其在免疫治疗中的研究进展[J].现代免疫学,2022,42(2):170-174.
[15] CAPETTI A F,OROFINO G,PALADINI L,et al.Does simplification to dolutegravir-based dual regimens impact on the CD4+/CD8+T cell ratio[J].AIDS,2018,32(8):1083-1084.
[16] WILDER-SMITH A,MUSTAFA F B,EARNEST A,et al.Impact of partial sleep deprivation on immune markers[J].Sleep Med,2013,14(10):1031-1034.
[17] 黄晓宇,谢光璟,李浩,等.天王补心丹加减对睡眠剥夺大鼠学习记忆及炎症因子表达的影响[J].中国实验方剂学杂志,2020,26(23):56-62.
[18] 任小娟,陈小兵,王冠英,等.D-半乳糖联合对氯苯丙氨酸致肾不藏志不寐大鼠肾脏氧化应激和炎性因子的实验研究[J].新疆中医药,2021,39(4):9-12.
[19] 高琼,刘昱昕,陈冬梅,等.氧化苦参碱调控FOXO1表达对TNF-α诱导HaCa T细胞炎症因子分泌的影响[J].广东医学,2022,43(1):40-45.
[20] 杨卫星,黄君,娄敏娟,等.Th1/Th2及其细胞因子在脓毒症患者中的改变和临床价值[J].中国现代医生,2021,59(16):56-59.
[21] 郑新伟,李冬,耿丽娟,等.慢性失眠伴轻度认知功能障碍患者血清细胞因子IL-4和IFN-γ的变化[J].中华行为医学与脑科学杂志,2020,29(8):700-705.
[22] 水玲,陈英松,阿古拉,等.慢性不完全性睡眠剥夺大鼠对海马TNF-α mRNA、IFN-γ mRNA表达及脾脏NK细胞活性的影响[J].辽宁中医杂志,2019,46(10):2209-2212.
[23] 洪宇泽,郑利平,刘飞,等.血清Th2型细胞因子IL-6、IL-10评估社区获得性肺炎病情及预后意义[J].中华肺部疾病杂志(电子版),2021,14(3):318-320.
[24] 尹超,游秋云,张美娅,等.生慧汤对慢性睡眠剥夺小鼠学习记忆及海马IL-6,TNF-α,COX-2基因表达的影响[J].中国实验方剂学杂志,2019,25(1):96-100.
[25] 常瑜,郝正伟,戈艳蕾,等.葡萄籽原花青素对中老年OSAHS 患者睡眠质量及血浆 IL-6和TNF-α水平的影响[J].新疆医科大学学报,2016,39(3):342-345.
[26] REN C Y,RAO J X,ZHANG X X,et al.Changed signals of blood adenosine and cytok- ines are associated with hparameters of sleep and/or cognition in the patients with chronic insomnia disorder[J].Sleep Med,2021,81:42-51.