水环境中3种典型污染物对海洋发光菌的联合毒性作用分析

职业与健康 ›› 2023, Vol. 39 ›› Issue (3): 380-383.

水环境中3种典型污染物对海洋发光菌的联合毒性作用分析

张建江¹, 刘建铃¹, 陈思萍²

1.宁德师范学院医学院，福建宁德 352100;
2.韶关学院医学院，广东韶关 512026

收稿日期:2022-07-08 修回日期:2022-07-18 发布日期:2026-03-02
通信作者: 陈思萍，教授，E-mail：xuyd1827@sina.com
作者简介:张建江，男，副教授，主要从事劳动卫生与环境卫生学研究工作。
基金资助:
宁德师范学院2019年第二批引进人才科研启动项目（2019Y19）; 2019年宁德市指导性科技计划项目（20190015）

Study on combined toxicity of three typical pollutants in water environment to marine luminescent bacteria

ZHANG Jian-jiang¹, LIU Jian-ling¹, CHEN Si-ping²

1. Medical College，Ningde Normal University，Ningde Fujian 352100，China;
2. Medical College，Shaoguan University，Shaoguan Guangdong 512026，China

Received:2022-07-08 Revised:2022-07-18 Published:2026-03-02
Contact: CHEN Si-ping，Professor，E-mail：xuyd1827@sina.com

摘要/Abstract

摘要： 目的分析水环境中3种典型污染物氰（CN^-）、汞（Hg²⁺）和草甘膦（Glyphosate）联合作用时对海洋发光菌的毒性。方法将不同浓度的CN^-、Hg²⁺和Glyphosate以相等体积两两组合配制成混合溶液，同时以3%NaCl溶液作为空白对照，加入明亮发光杆菌T3变种，分别用生物毒性测试仪测试样品和空白对照的发光度，计算发光损失率，并对数据进行分析。结果 CN^-和Hg²⁺联合作用时，不同浓度污染物组合所致的发光损失率之间的差异有统计学意义（P<0.01），其中，分别使发光菌的发光率降低50%的毒物浓度（ median effective concentration，EC₅₀）的CN^-和Hg²⁺组合所致的发光损失率最高，达到（84.00±16.74）%;CN^-和Glyphosate联合、Hg²⁺和Glyphosate联合作用时，也表现出类似的趋势（均P<0.01），浓度为EC₅₀的CN^-和Glyphosate组合所致的发光损失率为（81.07±17.22）%，浓度为EC₅₀的Hg²⁺和Glyphosate组合所致的发光损失率为（82.47±19.73）%。结论随着联合污染物浓度的提高，发光损失率也逐渐增高，二种污染物联合作用时对海洋发光菌所致的发光损失率大于二者的单独作用，利用海洋发光菌对水环境中联合污染物的毒性进行监测和评价具有一定优势。

关键词: 环境污染物, 联合毒性效应, 发光菌

Abstract: Objective To analyze the combined toxicity of three typical pollutants，including cyanogen（CN^-），mercury（Hg²⁺） and glyphosate，in water environment to marine luminescent bacteria. Methods The different concentrations of CN^-，Hg²⁺ and glyphosate were combined on pairwise mode in equal volumes to form a mixed solution. At the same time，3% NaCl solution was used as the blank control，and Photobacterium phosphoreum T3 variant was added. The luminosity of the samples and the blank control were tested with a biological toxicity tester，to calculate the luminescent loss rate，and the data were analyzed. Results There was statistically significant difference in the luminous loss rate caused by the combination of CN^- and Hg²⁺ at different concentrations（P<0.01）. The combination of CN^- and Hg²⁺ at the concentration of median effective concentration（EC₅₀），which reduced the luminescent rate of Photobacterium phosphoreum by 50%，resulted in the highest luminescent loss rate of （84.00±16.74）%. For the combinations of CN^- and glyphosate or Hg²⁺ and glyphosate，it also showed a similar trend（all P<0.01）. The luminous loss rate caused by the combination of CN^- and glyphosate at the concentration of EC₅₀ was （81.07±17.22）%，and the luminous loss rate caused by the combination of Hg²⁺ and glyphosate at the concentration of EC₅₀ was （82.47±19.73）%. Conclusions With the increase of the concentration of combined pollutants，the luminous loss rate also gradually increases. The luminous loss rate caused by the mixture of the two pollutants is greater than that caused by the two pollutants alone. Using marine luminescent bacteria to monitor and evaluate the toxicity of combined pollutants in water environment has certain advantages.

Key words: Environmental pollutants, Combined toxic effects, Luminescent bacteria

中图分类号:

R117

张建江, 刘建铃, 陈思萍. 水环境中3种典型污染物对海洋发光菌的联合毒性作用分析. [J]职业与健康, 2023, 39(3): 380-383.

ZHANG Jian-jiang, LIU Jian-ling, CHEN Si-ping. Study on combined toxicity of three typical pollutants in water environment to marine luminescent bacteria. [J]OCCUPATION AND HEALTH, 2023, 39(3): 380-383.

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