尿中多项生物监测指标的顶空气相色谱-质谱联用同时测定法

职业与健康 ›› 2023, Vol. 39 ›› Issue (4): 467-470.

尿中多项生物监测指标的顶空气相色谱-质谱联用同时测定法

何宗剑, 覃利梅, 朱定姬, 宁攀良

广西壮族自治区职业病防治研究院理化检测所,广西南宁 530021

收稿日期:2022-07-04 修回日期:2022-08-08 发布日期:2026-03-02
作者简介:何宗剑,男,工程师,主要从事理化检测工作。
基金资助:
广西壮族自治区卫生健康委员会课题（Z20211115）

Simultaneous determination of multiple biological monitoring indicators in urine by headspace gas chromatography-mass spectrometry

HE Zong-jian, QIN Li-mei, ZHU Ding-ji, NING Pan-liang

Department of Physical and Chemical Testing,Guangxi Zhuang Autonomous Region Academy for the Prevention and Treatment of Occupational Diseases,Nanning Guangxi 530021,China

Received:2022-07-04 Revised:2022-08-08 Published:2026-03-02

摘要/Abstract

摘要： 目的建立尿中丙酮、1-溴丙烷、二氯甲烷、2,5-己二酮、邻甲酚及苯酚等生物监测指标的顶空气相色谱-质谱联用测定方法。方法尿样加入无水硫酸钠后密封,经顶空进样,气相色谱质谱检测。结果该方法测定尿中各组分在各浓度范围内线性良好,相关系数为0.999 5~0.999 9,方法检出限在0.4~20.0 μg/L,样品加标回收率为84.7%~115.6%,相对标准偏差为2.6%~8.9%。结论该方法简便、准确、灵敏高,适用于化学有害因素的生物监测和中毒应急样品的快速检测。

关键词: 顶空, 气相色谱质谱, 尿, 生物监测

Abstract: Objective To establish a headspace gas chromatography-mass spectrometry（GC-MS） method for the determination of biological monitoring indicators in urine,such as acetone,1-bromopropane,dichloromethane,2,5-hexanedione,o-cresol and phenol. Methods The urine sample was sealed after adding anhydrous sodium sulfate,and was determined by GC-MS with headspace injection. Results The linearity of each component in urine determined by this method was good within each concentration range,the correlation coefficients were between 0.999 5 and 0.999 9,the detection limit was 0.4-20.0 μg/L,the sample recovery rate was 84.7%-115.6%,and the relative standard deviations were between 2.6% and 8.9%. Conclusion This method is simple,accurate,and highly sensitive,which is suitable for biological monitoring of occupational exposure to chemical harmful factors,and rapid detection of poisoning emergency samples.

Key words: Headspace, Gas chromatography-mass spectrometry, Urine, Biological monitoring

中图分类号:

R135.1

何宗剑, 覃利梅, 朱定姬, 宁攀良. 尿中多项生物监测指标的顶空气相色谱-质谱联用同时测定法. [J]职业与健康, 2023, 39(4): 467-470.

HE Zong-jian, QIN Li-mei, ZHU Ding-ji, NING Pan-liang. Simultaneous determination of multiple biological monitoring indicators in urine by headspace gas chromatography-mass spectrometry. [J]OCCUPATION AND HEALTH, 2023, 39(4): 467-470.

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